Genechip technology was used to analyze the expression profile of differential genes in corn treated by metabolites of endophyte under 10% PEG osmotic stress. The results showed that there were 441 differentially expressed genes identified in this study including 147 up-regulated genes and 294 down-regulated genes,participating in 21 metabolic pathways. The genes with differential expressions were classified by GO-ranking methods through CapitalBio Molecule Annotation System V 3.0(MAS). Among them 45% participated in various biological processes,18% were related to synthesis of cellular components,the coded products of 36% genes were related to performing a variety of molecular function,and 1% corresponded with other unknown sequences in GenBank. Thus the role of these genes still needs further study. Pathways analysis using the KEGG pathway database showed the differentially expressed genes broadly were involved in basic metabolism, energy metabolism,secondary metabolism and signal transduction pathways. The expression profile analysis indicate that the effects of secondary metabolites on corn under osmotic stress are synergistical processes by many genes in many pathways.%利用基因芯片技术对10% PEG 渗透胁迫下植物内生菌次生代谢产物处理的玉米进行差异基因表达谱分析。结果显示，检测到的差异表达基因共计441个，其中上调表达基因147个，下调表达基因294个，参与21条代谢通路。采用晶芯生物分子功能注释系统 V 3.0（MAS）将差异表达基因进行 GO 功能分类，其中45%基因参与各种生物学过程；18%基因与生成各种细胞组分有关；36%基因编码产物则与执行各种分子功能有关；1%与 GenBank 中功能未知序列相对应。KEGG 代谢分析表明差异表达基因广泛涉及基础物质代谢、能量代谢、次生代谢及信号转导途径。表达谱分析结果表明，在渗透胁迫下次生代谢产物对玉米的影响是一个多基因参与、多个生物途径协同调控的过程。
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