In this article,ion-pair chromatography with ultraviolet detection was used in the analysis of p-toluenesulfonate. The ana-lyte was separated using a mobile phase of tetrabutylammonium hydroxide( TBA)-citric acid-acetonitrile on a reversed-phase silica-based monolithic column. The ultraviolet detection wavelength,the composition of mobile phase,column temperature and flow rate on the ion retention and separation were investigated,retention rule was discussed. The chromatographic conditions were optimized:at 230 nm wavelength,when 0. 4 mmol·L-1 TBA-0. 13 mmol·L-1 citric acid-15%(v/v)acetonitrile(pH 5. 5)was used as mobile phase with column temperature of 40℃ and flow rate of 3. 0 mL·min-1 ,retention time of C7 H7 SO3-was less than 1. 25 min. Common inor-ganic anions( Cl-,NO3-) did not interfere with the determination. In the range of 0. 7-100 mg·L-1 ,the correlation coefficient of line-ar regression equation was 0. 9997. Relative standard deviation(RSD)of chromatographic peak area was 1. 0%(n=5). Detection limit(S/N=3)was 0. 2 mg·L-1. The method has been applied to the determination of p-toluenesulfonate ionic liquid samples syn-thesized by chemistry laboratory. Standard addition recovery rate was 101%. The method was simple and rapid.%研究了整体柱离子对色谱结合紫外检测的方法分析对甲苯磺酸根( C7 H7 SO3-)。采用反相硅胶整体柱,以氢氧化四丁基铵-柠檬酸-乙腈为流动相,考察了紫外检测波长、流动相组成、色谱柱温度和流速对离子保留的影响。色谱条件经过优化,在紫外检测波长230 nm,流动相为0．4 mmol·L-1氢氧化四丁基胺( TBA)-0．13 mmol·L-1柠檬酸-15%(v/v)乙腈(pH 5．5),流速3．0 mL·min-1,柱温40℃的条件下,C7H7SO3-的保留时间为1．25 min内。常见无机阴离子( Cl-,NO3-)不干扰测定。在0．7~100 mg·L-1范围内,线性回归方程的相关系数为0．9997,峰面积的相对标准偏差(RSD,n=5)为1．0%,检出限(S/N=3)为0．2 mg·L-1。该方法应用于检测实验室合成离子液体中的C7 H7 SO3-,加标回收率为101%。方法简单、快速。
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