本文研究了复合酶酶解前后假酸浆提取物的抗氧化活性能力，并采用薄层色谱法（ TLC）对酶解产物中总黄酮进行了分离，利用高效液相色谱-质谱联用技术对其鉴定分析。通过对DPPH自由基、羟自由基及超氧阴离子自由基清除能力实验，酶解液抗氧化性能显著高于假酸浆提取液；酶解液的薄层及HPLC-MS法初步鉴定出假酸浆中含有桑色素、二氢槲皮素和儿茶素三种黄酮。%The enzymatic hydrolyzing condition was studied for flavonoids from Nicandra Physaloides .Flavonoid composition analysis was achieved by using TLC and HPLC-MS.According to experiment on free radical scavenging activity ,that antioxidativity of sam-ples incrdased in different degree after enzyme action.TLC and HPLC-MS shows four kinds of flavonoid aglycone:Morin、Taxifolin and Catechin.
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