To determine the roles of miRNA in the developmental stages of Xiang pig,we investigated the miRNA expression patterns in different months old of Xiang pigs.The total RNA from 3 and 7 months old of Xiang pigs were collected separately.Then,the total RNA were hybridized with the miRNA microarray and the threshold (fold-change>2, P<0.05) were used to identify the different expression miRNA.The quantitative Real-time PCR was performed to verify the microarray result.Compared to the 3 months old group,there were 11 miRNA down regulated and 9 miRNA were up regulated in the 7 months old group.The lowest down regulated miRNA was miR-27b whereas the highest up regulated one was miR-155.The result of quantitative Real-time PCR consistented with the microarray result.The result suggested that some of the dysregulated expression of miRNA might participate in the lipid metabolism in hepatic cells.%为研究miRNA在香猪发育时期的调控功能,试验对3和7月龄香猪肝脏组织中存在表达差异的miRNA进行了筛选.提取3和7月龄香猪肝脏组织总RNA,通过与miRNA芯片杂交,经统计学分析,以变化倍数≥2倍(P<0.05)为阈值,筛选3和7月龄香猪肝脏中表达丰度差异的miRNA,并运用实时荧光定量PCR进行验证.结果发现,与3月龄相比,7月龄香猪肝脏中有11条miRNA表达下调和9条miRNA表达上调,下调倍数最多的是miR-27b,上调倍数最多的是miR-155.对选取的4条差异表达的miRNA进行实时荧光定量PCR反应,结果显示,4条miRNA表达变化趋势与芯片结果一致.本研究结果表明,不同月龄香猪肝脏中的miRNA表达丰度有差异,其中一些可能参与了机体生长发育时期肝细胞的脂肪代谢过程.
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