目的:比较实时荧光定量-PCR和酶联免疫吸附法检测巨细胞病毒-IgM抗体水平的敏感性及准确率.方法:收集108例孕前筛查妇女,同时采用实时荧光定量-PCR和酶联免疫吸附法检测外周血巨细胞病毒-IgM抗体水平,并对这两种检测方法的阳性率进行比较.结果:实时荧光定量-PCR法阳性率为74%,酶联免疫吸附法为57%,两者差异有统计学意义(P<0.05);18例荧光定量-PCR法阳性的妇女血清巨细胞病毒-IgM拷贝数低于10-4/ml.结论:实时荧光定量-PCR与酶联免疫吸附法相比,对巨细胞病毒-IgM抗体检测具有较高的敏感性和准确率,值得临床推广使用.%Objective: To compare the sensitivity and accuracy of cytomegalovirus-IgM (CMV-IgM) detection by real-time PCR and enzyme linked immunoabsorption assay (ELISA). Methods: We enrolled 108 females who were underwent pre-pregnancy evaluation and evaluated peripheral blood CMV-IgM level by both real time-PCR and ELISA. Positive rates of these two methods were compared. Results: Positive rate in real time-PCR was 74％ while in ELISA was 57％, and there was a significant difference between these two methods (ρ＜0.05). Copy number in 18 positive cases with real-time PCR detection was less than l0-4/ml. Conclusion: The sensitivity and accuracy of CMV-IgM detection with real-time PCR is higher than ELISA, and real-time PCR deserves broadly applied in clinic.
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