目的:建立黄芩片中黄芩苷的含量测定方法.方法:采用HPLC法,色谱柱为ODS C18柱(5 μm,4.6 mm×150 mm),流动相为甲醇-水-磷酸溶液(47:53:0.2),流速为1.0 ml/min,检测波长为280 nm.结果:黄芩苷进样浓度在9.4～188.0 μg/ml(r=0.999 7)范围内线性良好,平均回收率为99.6%,RSD为1.32%(n=6).结论:本文建立的HPLC法可作为黄芩片的质量控制方法.%Objective: To develope a method for determining baicalin in Huangqin Tablets. Methods: The ODS C18 col umn (5 μm, 4.6 mm×150 mm) was used, the mobile phase was methanol-water-phosphoric acid (47:53:0.2) at the flow rate of 1.0 ml/min, the detective wavelength was 280 nm. Results: The linear range of baicalin concentration was 9.4-188.0 μg/ml (r=0.999 7), the average recovery was 99.6% with a RSD of 1.32% (n=6). Conclusion: The method is simple, fast and ac-curate. It can be used for the quality control of Huangqin Tablets.
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