The easily oxidized docosahexaenoic acid (DHA) was protected by solid lipid nanoparticles ( SLN) . Glyceryl monostearate was used as wall material and DHA algal oil was used as core material to prepare DHA - SLN by high pressure homogenization. The technological parameters were optimized by response surface methodology on the basis of single factor experiment with DHA - SLN embedding rate as response value. The optimal technological parameters were as follows: ratio of core material to wall material 1: 65, homogenization pressure 80 MPa, mass fraction of emulsifier 2. 14% (based on aqueous phase), homogenization times 8. Under these conditions, the embedding rate of DHA - SLN was 74. 45%.%采用固体脂质纳米粒保护容易氧化的二十二碳六烯酸(DHA).以单硬脂酸甘油酯为壁材,DHA藻油为芯材,采用高压均质制备DHA固体脂质纳米粒(DHA-SLN).以DHA-SLN的包封率为指标,通过单因素实验和响应面法优化工艺参数,得出高压均质法制备DHA-SLN的最佳工艺条件是:芯壁比1∶65,均质压力80 MPa,乳化剂质量分数2.14％(占水相),均质8次.在此条件下,实验验证的DHA-SLN的包封率为74.45％.
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