Objective To learn the effect of strontium doped calcium phosphate bone repair material on VEGF protein secretion in os-teoblasts and to provide a new bone repair material for orthopedics clinic. Methods The strontium doped calcium phosphate bone re-pair materials with different strontium content were prepared. The VEGF protein secretion was measured by ELISA method, and the ef-fect of MTT on the proliferation of osteoblasts was investigated. Results The results of ELISA method after 7 d showed that the secre-tion of VEGF in osteoblasts of 1%, 3%, 5%, 8%, 10% strontium doped calcium phosphate were higher than calcium phosphate, and the differences were statistically significant ( t=6. 561, 11. 454, 11. 256, 15. 149, 9. 778, P ﹤ 0. 05 ) . Among them, the content of VEGF was the highest in the 8% calcium containing calcium strontium calcium phosphate. The results of MTT method showed that the A values of 3, 5, 7, 9 d was higher than that of the negative control, and the value of the osteoblasts was higher than that of the negative control, and the differences were statistically significant ( t=3. 960, 5. 029, 5. 229, 4. 808, 2. 584, 2. 954, 2. 657, 2. 798, P ﹤ 0. 05 ) . After culture 5, 7, 9 d, the A value of the osteoblast of the 8% calcium doped calcium phosphate solution were higher than that of calcium phos-phate, and the differences were statistically significant ( t=2. 583, 2. 930, 1. 934, P ﹤ 0. 05 ) . Conclusion As a new bone repair materi-al, strontium doped calcium phosphate can promote the secretion of VEGF, which has a good clinical application prospects.%目的：探讨掺锶聚磷酸钙骨修复材料对成骨细胞血管内皮生长因子（VEGF）蛋白分泌量的影响。方法制备不同含锶量的掺锶聚磷酸钙骨修复材料，采用酶联免疫吸附（ELISA）法测定成骨细胞的VEGF蛋白分泌量，并采用噻唑蓝( MTT )法考察其对成骨细胞增殖的影响。结果 ELISA法结果表明，培养7 d后，1%，3%，5%，8%，10%含锶量的掺锶聚磷酸钙作用的成骨细胞VEGF分泌量均高于聚磷酸钙，差异均有统计学意义( t=6.561，11.454，11.256，15.149，9.778，P﹤0.05)，其中，8%含锶量掺锶聚磷酸钙作用的成骨细胞VEGF分泌量最高。MTT法结果表明，培养3，5，7，9 d后，8%掺锶聚磷酸钙、聚磷酸钙浸提液作用的成骨细胞的吸光度（ A）值高于阴性对照，差异均有统计学意义( t=3.960，5.029，5.229，4.808，2.584，2.954，2.657，2.798，P﹤0.05)；培养5，7，9 d后，8%掺锶聚磷酸钙浸提液作用的成骨细胞的 A值高于聚磷酸钙，差异均有统计学意义( t=2.583，2.930，1.934，P﹤0.05)。结论掺锶聚磷酸钙作为一种新型的骨修复材料，能促进成骨细胞分泌VEGF，具有良好的临床应用前景。
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