目的:研究γ-氨基丁酸A(GABAA)受体与七氟烷(S)致幼小鼠遗忘作用和自主活动抑制作用的关系.方法:取幼小鼠随机分为3大组(n=80),分别进行自主活动、跳台和避暗实验,再将每大组幼小鼠随机分为空白对照组(生理盐水10ml/kg),S组(3.0 ml/kg),GABAA受体特异性阻滞药一叶荻碱(Se)低、中、高剂量组(2、4、8mg/kg)及其联用低、中、高剂量组(S3.0 ml/kg+Se 2、4、8 mg/kg),每组10只.实验开始时予幼小鼠腹腔注射Se或生理盐水,10 min后皮下注射S,记录末次给药后15、30、45、60 min时自主活动实验幼小鼠5 min内的自主活动次数,末次给药后1、2d时跳台和避暗实验步入潜伏期、跳下潜伏期和错误次数.结果:与空白对照组比较,S组幼小鼠给药后15、30、45 min时的自主活动次数明显减少,给药后1d的步入潜伏期、跳下潜伏期均明显缩短,其相应错误次数均明显增加(P＜0.05);联用低、中、高剂量组幼小鼠给药后15 min时的自主活动次数明显减少(P＜0.05).与S组比较,联用高剂量组幼小鼠给药后1d的步入潜伏期、跳下潜伏期均明显延长,其相应错误次数均明显减少(P＜0.05).其余各组指标间差异无统计学意义(P＞0.05).结论:GABAA受体参与了S的遗忘作用,但S对自主活动的抑制作用与GABAA受体无关.%OBJECTIVE:To investigate relationship of γ-aminobutyric acid type A (GABAA) receptor with sevoflurane (S)-induced spontaneous motor activity inhibition and amnesia in juvenile mice.METHODS:Juvenile mice were randomly divided into 3 groups (n=80) and included in spontaneous motor activity test,step-down test and step-through test.Each group was subdivided into blank control group (NS 10 ml/kg),S group (3.0 ml/kg),special GABAA receptor blocker Securinine (Se) low-dose,medium-dose and high-dose groups (2,4,8 mg/kg),drug combination low-dose,medium-dose and high-dose groups (S 3.0 ml/kg+Se 2,4,8 mg/kg) with 10 mice in each group.The mice received intraperitoneal injection of Se or NS at the beginning of experiment and 10 min later received hypodermic injection of S.The times of spontaneous motor activity within 5 min were recorded 15,30,45 and 60 min after last administration,and the step-through latency,step-down latency and error times of step-down test and step-through test were recorded 1 d and 2 d after last administration.RESULTS:Compared with blank control group,the times of spontaneous motor activity in S group decreased significantly 15,30 and 45 min after medication,and step-through latency and step-down latency shortened significantly 1 d after medication,while error times increased significantly (P＜0.05) ; the times of spontaneous motor activity in drug combination low-dose,medium-dose and high-dose groups decreased significantly 15 min after medication (P＜0.05).Compared with S group,step-through latency and step-down latency prolonged significantly in drug combination high-dose group 1 d after medication,while the error times decreased significantly (P＜0.05).Other index of each group had no statistical significance (P＞0.05).CONCLUSIONS:S can also induce amnesia,and GABAA receptor is involved in this effect.S can decrease the times of spontaneous motor activity,but GABAA receptor isn' t involved in this effect.
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