Agrobacterium tumefaciens mediated transformation ( ATMT ) was applied in this research. Agrobacterium tumefaciens, which carried plasmid PCH-sGFP harboring the green fluorescent protein (gfp) gene and hygromycin phosphotransferase gene ( hph), was used to transform Fusarium oxysporum f.sp. Conglutinans race 1 and 2. The transformants were randomly selected to confirm. The results indicated that transformants were integrated with exogenous T-DNA in genomes successfully, and gfp gene was stable after 6 successive subcultures. The growth rates analysis and pathogenicity showed that there were no significant differences between wild isolates and transformants on growth rates and pathogenicity. So transformant can be used as a useful tool for further function research, such as histopathology and pathogenesis.%采用根癌农杆菌介导的方法,以农杆菌介导丝状真菌遗传转化的载体PCH-sGFP对尖孢镰刀菌甘蓝专化型两个生理小种进行转化,该载体含有报告基因—绿色荧光蛋白基因(gfp)和筛选基因—潮霉素磷酸转移酶基因(hph).获得转化菌株后,经检测表明:T-DNA目的片段成功整合到枯萎病菌基因组中,并且单孢继代培养6代后荧光蛋白仍能稳定遗传.对转化菌株的生长速度和致病力进行分析,结果表明:野生菌株和转化菌株在生长速度和致病力上没有显著差异.因此,转化菌株可用于甘蓝枯萎病菌的组织病理学研究.
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