Objective To investigate the relation between Vav3 expression and tumor invasion and metastasis in gastric cancer tissue and the clinical significance. Methods We collected the paraffin specimens of gastric cancer tissue and carcinoma side normal tissue from 92 patients who received resection for gastric carcinoma in the Third Surgery Department of the Fourth Affiliated Hospital of Hebei Medical University from May 2014 to May 2015. The expressions of Vav3,RelA,PCNA and MMP-2 in gastric cancer tissue and carcinoma side normal tissue were detected by immunohistochemistry staining. The relation between Vav3 expression and clinical and pathological characteristics in gastric cancer tissue was analyzed. The relation among Vav3, RelA,PCNA and MMP-2 expression was investigated by Spearman correlation analysis. Results The positive expression rate of Vav3 in gastric cancer tissue was 78. 3% (72 / 92),higher than 40. 2% (37 / 92)in carcinoma side normal tissue( χ2 = 27. 572,P ﹤ 0. 001). The positive expression rate of RelA in gastric cancer tissue was 88. 0% (81 / 92),higher than 52. 2% (48 / 92)in carcinoma side normal tissue(χ2 = 28. 242,P ﹤ 0. 001). The positive expression rate of PCNA in gastric cancer tissue was 73. 9% (68 / 92),higher than 47. 8% (44 / 92)in carcinoma side normal tissue(χ2 = 13. 143,P ﹤ 0. 001) . The positive expression rate of MMP-2 in gastric cancer tissue was 69. 6% ( 64 / 92 ),higher than 48. 9% ( 45 / 92 )in carcinoma side normal tissue( χ2 = 8. 125,P = 0. 004 ) . There was no significant difference in gender,age,depth of infiltration,and distant metastasis between high expression of Vav3 and low expression of Vav3 ( P ﹥ 0. 05 ) . There was significant difference in tumor differentiation degree,tumor diameter and the extent of lymph node metastasis between high expression of Vav3 and low expression of Vav3(P ﹤ 0. 05). The expression of Vav3 had positive correlation with the expression of RelA,PCNA and MMP-2(rs = 0. 338,P ﹤ 0. 001;rs = 0. 225,P = 0. 031;rs = 0. 267,P = 0. 010). The expression of RelA had positive correlation with the expression of PCNA( rs = 0. 336,P = 0. 001);the expression of RelA had no linear correlation with the expression of MMP-2(rs = 0. 176,P = 0. 094). The expression of PCNA had no linear correlation with the expression of MMP-2(rs = 0. 188,P = 0. 072). Conclusion There is overexpression of Vav3 in gastric cancer tissue,and the increase of Vav3 is associated with tumor metastasis and progress. Vav3 is involved in the progress and metastasis of gastric cancer by regulating RelA,PCNA and MMP-2 expression.%目的：探讨胃癌组织中 Vav3表达与肿瘤侵袭转移的关系及其临床意义。方法选取2014年5月—2015年5月于河北医科大学第四医院外三科住院并行肿瘤切除的胃癌患者92例。收集患者胃癌组织及癌旁正常组织（距肿瘤边缘﹥3 cm，镜检无癌细胞）的石蜡标本，采用免疫组化法检测 Vav3、RelA、增殖细胞核抗原（PCNA）、基质金属蛋白酶2（MMP-2）在胃癌组织及癌旁正常组织中的表达；分析胃癌组织中 Vav3表达强度与临床病理特征的关系；Vav3、RelA、PCNA、MMP-2表达之间的关系采用 Spearman 秩相关分析。结果胃癌组织中 Vav3的阳性表达率为78.3％（72/92），高于癌旁正常组织的40.2％（37/92）（χ2＝27.572，P ﹤0.001）；胃癌组织中 RelA 的阳性表达率为88.0％（81/92），高于癌旁正常组织的52.2％（48/92）（χ2＝28.242，P ﹤0.001）；胃癌组织中 PCNA 的阳性表达率为73.9％（68/92），高于癌旁正常组织的47.8％（44/92）（χ2＝13.143，P ﹤0.001）；胃癌组中MMP-2的阳性表达率为69.6％（64/92），高于癌旁正常组织的48.9％（45/92）（χ2＝8.125，P ＝0.004）。Vav3强表达患者性别、年龄、浸润深度、远处转移情况与 Vav3弱表达比较，差异无统计学意义（P ﹥0.05）；Vav3强表达患者肿瘤分化程度、肿瘤直径、淋巴结转移程度与 Vav3弱表达比较，差异有统计学意义（P ﹤0.05）。Vav3表达与 RelA、PCNA、MMP-2表达均呈正相关（rs ＝0.338，P ﹤0.001；rs ＝0.225，P ＝0.031；rs ＝0.267，P ＝0.010）；RelA 表达与 PCNA 表达呈正相关（rs ＝0.336，P ＝0.001）；RelA 表达与MMP-2表达无直线相关关系（rs ＝0.176，P ＝0.094）；PCNA 表达与MMP-2表达无直线相关关系（rs ＝0.188，P ＝0.072）。结论胃癌组织中 Vav3存在过表达现象，Vav3表达增强与肿瘤转移及进展有关。Vav3可能通过调节 RelA、PCNA、MMP-2表达而参与胃癌侵袭转移。
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