Objective To study the methylation status of RASSF1A gene promoter in cervical carcinoma and explore its clinical significance. Methods Microspectrophotometry ( MSP ) and reverse transcription polymerase chain reaction ( RT -PCR ) were performed to detect the methylation status of RASSF1A gene promoter and its mRNA expressions in cervical carcinoma and paracancerous tissues in 65 patients with cervical carcinoma, which was confirmed in our hospital from Jan 2008 to Jan 2009.Results Of these 65 cervical carcinoma tissues, methylated strap ( methylation rate : 73. 8％ ) with inhibited mRNA expression were ohserved in 48 patients. Four patients had methylated promoters in paracancerous tissues with normal mRNA expression.The methylation of RASSFIA gene promoter in cervical carcinoma was not correlated with age ( P ＞ 0. 05 ) , but was positively correlated with tumor diameter ( P ＜0. 05 ). The methylation rate of RASSF1A gene promoter in cervical squamous cell carcinoma. adenocarcinoma, and adenosquamous carcinoma were 82. 0％ , 40. 0％ , and 60. 0％, respectively ( P ＜ 0. 05 ). The methylation of RASSFIA gene promoter in cervical carcmoma was positively correlated with FIGO staging and lymph node metastasis ( P ＜ 0. 05 ). Conclusion RASSFIA gene promoter hypermethylation may play a crucial role in the pathogenesis of cervical carcinoma. It is positively correlated with clinical staging and pathologic types. It can be served as a tumor hiomarker in the molecular diagnosis and prognosis evaluation of cervical carcinoma.%目的 检测宫颈癌RAS相关区域家族1A(RASSF1A)基因启动子甲基化状态并探讨其在宫颈癌分子诊断与预后评估中的临床意义.方法 应用甲基化特异性PCR和逆转录PCR分别检测我院2008年1月-2009年1月确诊的65例宫颈癌组织和癌旁正常组织RASSF1A基因启动子甲基化状态和mRNA表达,比较两者间的差异与临床病理因素之间的关系.结果 65例宫颈癌组织中有48例检测出甲基化条带(甲基化率73.8%),mRNA表达呈抑制状态,癌旁组织中有4例检测出甲基化条带(甲基化率6.2%),mRNA表达正常;不同年龄的患者RASSF1A基因启动子甲基化率间差异无统计学意义(P＞0.05);肿瘤直径越大,RASSF1A基因启动子甲基化率越高;宫颈鳞状细胞癌、腺癌和腺鳞癌RASSF1A基因启动子甲基化率分别为82.0%、40.0%和60.0%,三者间差异有统计学意义(P＜0.05);宫颈癌RASSF1A基因启动子甲基化率随着临床分期和淋巴结转移增加而增加,甲基化率在不同国际妇产科联盟(FIGO)分期及有无淋巴结转移中的差异均有统计学意义(P＜0.05).结论 RASSF1A基因启动子甲基化与宫颈癌的发病机制紧密相关,其在宫颈鳞状细胞癌中甲基化发生率明显高于腺癌,可作为宫颈癌的分子诊断和预后评估的生物标志物.
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