目的：探讨凋亡调控因子Fas、FasL及caspase-3在肝细胞癌（HCC）组织中的表达及其临床意义。方法应用免疫组织化学SP法检测80例HCC患者肝癌组织及其相应癌旁正常肝组织中Fas、FasL及caspase-3的表达，并分析其表达与 HCC临床病理因素的相关性。结果 Fas、FasL及caspase-3在 HCC、癌旁组织中的表达比较，均差异有统计学意义（P＜0．05）。在TNM 分期（Ⅲ～Ⅳ期）、有门静脉癌栓、有淋巴结转移的肝癌组织中Fas 的阳性表达率明显降低（P＜0．05）；在病理分级（Ⅲ～Ⅳ级）、TNM分期（Ⅲ～Ⅳ期）、有门静脉癌栓、有淋巴结转移的肝癌组织中FasL的阳性表达率明显增高（P＜0．05）；在病理分级（Ⅲ～Ⅳ级）、TNM分期（Ⅲ～Ⅳ期）、有淋巴结转移的肝癌组织中 caspase-3的阳性表达率明显降低（P＜0．05）。80例 HCC患者肝癌组织中Fas、FasL与caspase-3的表达，两两之间并无明显关联性（狉＝0．057，P＞0．05）。结论 Fas/FasL系统的失衡及caspase-3低表达导致肝癌细胞凋亡障碍，在 HCC发生、发展及转移中起着重要作用。%Objective To investigate the expression and clinical significance of apoptosis regulatory factors Fas, FasL and caspase-3 in hepatocellular carcinoma (HCC)tissues. Methods SP immunohistochemistry was used to detect the expression of Fas,FasL and caspase-3 in HCC tissues and the corresponding para-tumor normal liver tissues of 80 cases. The correlation between the expression and clinical pathological factors of HCC was analyzed.Results Compared to para-tumor tissues,the expression of Fas,FasL and caspase-3 in HCC was statistically significant difference (t=2.34,3.53,6.28,respectively,P<0.05). The positive expression rate of Fas was significantly reduced in HCC tissues of TNM staging Ⅲ~Ⅳ with portal vein tumor thrombus and lymphatic metastasis (t= 6.65,P<0.05). There was an obvious increase of positive expression rate of FasL in HCC tissues of pathological grading Ⅲ~Ⅳand TNM staging Ⅲ~Ⅳwith portal vein tumor thrombus and lymphatic metastasis (t= 7.13,P<0.05). Besides,a significant decrease of the positive expression rate of caspase-3 was observed in HCC tissues of pathological grading Ⅲ~Ⅳ and TNM staging Ⅲ~Ⅳwith lymphatic metastasis (t= 3.96,P<0.05). No obvious correlation existed between any two of the three factors (r= 0.057,P>0.05).Conclusions he imbalance of Fas/FasL systems and lower expression of caspase-3 may contribute to the apoptosis disorder of HCC cells,which plays an important role in the occurrence,development and metastasis of HCC.
展开▼
