本试验旨在研究肉种鸡补充硒代蛋氨酸对后代肉鸡肉质的影响,并从肌肉组织的硒沉积、抗氧化功能、组织形态、谷胱甘肽过氧化物酶(GSH-Px)基因表达方面探讨其作用机理.试验选用39周龄岭南黄肉种鸡240只,随机分为2组,每组3个重复,每个重复40只.试验设亚硒酸钠组(SS组)和DL-硒代蛋氨酸组(SM组),均在基础饲粮中(硒的实测值为0.04 mg/kg)添加硒0.3 mg/kg.种蛋按重复分别孵化后,初生雏鸡每组分3个重复,每重复各选60只.后代肉鸡饲喂不加硒的相同饲粮56 d.结果表明:同比SS组,DL-硒代蛋氨酸使后代肉鸡胸肌宰后48 h滴水损失率绝对值降低了0.90%(P<0.05);极显著地提高了后代肉鸡肌肉组织、血清及种蛋的硒含量(P<0.01);提高了1日龄雏鸡肌肉GSH-Px(P<0.05)、超氧化物歧化酶(SOD)活性(P<0.01)、总抗氧化能力(T-AOC)(P<0.05);增强了56日龄后代肉鸡血清和肌肉GSH-Px活性(P<0.01)、血清SOD活性(P<0.05)、肌肉T-AOC(P<0.05);使后代肉鸡腿肌肌丝整体排列均匀致密;并使后代肉鸡胸肌的GSH-Px mRNA表达增高了16.26%(P<0.05).结果提示:肉种鸡补充硒代蛋氨酸能显著提高种蛋及后代肉鸡肌组织的硒沉积量,促进相关抗氧化酶的基因表达,增强其抗氧化系统功能,有助于维持肌细胞的完整性,从而达到提高肉质的作用效果.%This experiment was conducted to study the effects of maternal dietary DL-selenomethionine on meat quality of progenies of broiler breeders and to investigate its mechanism through selenium ( Se) deposition, antioxidant capacity, histological morphology and gene expression of glutathione peroxidase ( GSH-Px) in muscle.A total of 240 Lingnan yellow broiler breeders of 39 weeks of age were randomly allocated to 2 groups, each group contained 3 replicates of 40 birds. Chickens in two groups were fed the same basal diet containing 0. 04 mg/kg Se, and supplemented with 0.3 mg/kg Se in the form of sodium selenite ( SS) and DL-selenomethionine ( SM) , respectively. Eggs were collected by each replicate and then hatched. Sixty new-born chicks of each replicate were selected. The total 360 chicks were fed the same basal diet containing 0. 04 mg/kg Se, without additional Se supplementation for 56 days. The results showed that compared with SS, SM decreased the drip loss of breast muscle by 0. 90％ (P ＜0. 05) at 48 h after slaughter in pregenies of 56-day-old, and significantly increased Se concentration in eggs, serum and muscle of pregenies ( P ＜0. 01) . SM also increased more total antioxidant capacity (T-AOC) ( P ＜ 0. 05) , GSH-Px ( P ＜ 0. 05) and superoxide dismutase ( SOD) activities (P ＜0. 01) in muscle of 1-day-old chicks than SS. As to the 56-day-old broilers, SM was more effective than SS in enhancing the activity of serum SOD (P ＜0. 05) , serum and muscle GSH-Px activities (P ＜0. 01) and muscle T-AOC (P ＜0.05) , and increasing the mRNA expression of GSH-Px in leg muscle by 16. 26％ (P ＜0. 05) . In addition, maternal SM induced the regular arrangement of myofilament of leg muscle in pregenies. In conclusion, it suggests that maternal SM, compared with SS, significantly increases Se levelin eggs, promotes the deposition of Se in serum and breast muscle of progenies, and enhances the antioxidant capacity in muscle and the gene expression of related antioxidant enzymes. SM also maintains the integrity of muscle cells and improves meat quality of broilers. [ Chinese Journal of Animal Nutrition ,2011 ,23 ( 3) :417-425 ]
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