To study vitamin D3 regulation of β-defensins genes in the different tissues of Silky Fowl, real-time quantitative PCR method was used to analyze the expression of AVBD-1, GAL-1 and AVBD-5 in thymus, duodenum, jejunum, ileum, cecum and bursa F.of Silky Fowl with fed different levels of vitamin D3(800, 1 600, 3 200 and 6 400 IU/kg).The results showed that three β-defensins gene expressions were found in six tissues of Silky Fowl, and the highest gene expression levels of AVBD-1 and GAL-1 were both in jejunum of Silky Fowl which fed 3 200 IU vitamin D3 ; the highest gene expression level of AVBD-5 in duodenum of Silky Fowl was in group fed 1 600 IU vitamin D3; in the tissues of Silky Fowl fed 800, 1 600, 3 200 and 6 400 IU vitamin D3 per kg diet, respectively, expression of the three AVBDs genes were directly up-regulated, then rapidly down-regulated.The results indicated that a dose-dependent manner that the gene expression levels in same tis-sues and vitamin D3 supplementary levels in diet of Silky Fowl was seen, and the suitable level of vitamin D3 could directly up-regu-late the gene expression of β-defensins in tissues of Silky Fowl.%本试验旨在研究不同水平维生素D3对丝毛乌骨鸡组织中β-防御素(AVBDs)基因表达的调控.采用实时荧光定量PCR方法检测饲喂不同水平(800、1 600、3 200和6 400 IU/kg)维生素D3对丝毛乌骨鸡胸腺、十二指肠、空肠、回肠、盲肠和法氏囊中3个β-防御素(AVBD-1、AVBD-5和GAL-1)基因表达水平的影响.结果表明:3个β-防御素基因在6个组织中均有表达,其中乌骨鸡AVBD-1和GAL-1基因的最高表达水平都在采食了3 200 IU/kg维生素D3时的空肠;AVBD-5基因的最高表达水平在采食了1 600 IU/kg维生素D3时的十二指肠;在依次采食维生素D3800、1 600、3 200和6 400 IU/kg的乌骨鸡组织中,3个β-防御素基因都存在先升后降的表达趋势.结果表明,β-防御素基因在同一组织的表达水平与维生素D3饲喂水平存在剂量关系,适宜的维生素D3饲喂水平能上调这3个β-防御素基因在乌骨鸡组织中的表达量.
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