To identify the Torque teno sus virus (TTSuV), a PCR-DHPLC assay was developed in this study. Primers specific for the partial region of the TTSuVl and TTSuV2 were selected to conduct the PCR-DHPLC assays. The specificity test was performed with PPV, PCV-H and PRV and no cross reaction was found. The PCR-DHPLC for TTSuV had good specificity and nice repeatability. Sensitivity analysis showed that the developed PCR-DHPLC could detect 1. 0×101 copy o μL-1. The established method and the Real-time PCR were used to detect 80 serum samples, the results showed that 63 samples were TTSuVl positive by PCR-DHPLC, and it was consistent with real-time PCR test; 69 samples were TTSuV2 positive by PCR-DHPLC, and it was consistent with real-time PCR test, 56 samples were positive by normal RT-PCR. The method showed nice specification, sensitivity, repeatability, and quickness, and it could be used in epide-miological investigation of TTSuV.%为了应用PCR结合变性高效液相色谱(DHPLC)技术检测猪细环病毒1型和2型,根据猪细环病毒的序列特点设计特异性引物,PCR扩增产物经DHPLC技术进行快速检测.选择猪细小病毒、猪圆环病毒Ⅱ型和猪伪狂犬病毒进行特异性试验,无交叉反应.该检测方法具有较好的特异性和重复性；用质粒标准品进行TTSuV1和TTSuV2的PCR-DHPLC检测,灵敏度可达1.0×101拷贝·μL-1.对80份血清样本分别应用实时荧光PCR法、PCR-凝胶电泳法及本文所建立的PCR-DHPLC法与进行检测,发现TTSuV1有63份PCR-DHPLC阳性,63份实时荧光PCR阳性,54份PCR凝胶电泳阳性；TTSuV2有69份PCR-DHPLC阳性,69份实时荧光PCR阳性,56份PCR-凝胶电泳阳性.本试验建立的PCR-DHPLC法具有特异、敏感、快速、重复性好等优点,可用于TTSuV感染的分子流行病学调查.
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