为了探讨Y-box结合蛋白(YB1)是否参与哺乳动物的冷适应反应,本研究采用RT-PCR和Real-time PCR 的方法克隆测序了民猪YB1基因的序列,检测了该基因在大肠、腿肌、肺脏、脂肪、心脏、脾脏和肝脏组织的表达情况,比较了该基因在冷刺激13 d后常温组((10±2)℃)和冷刺激组((-20±3)℃)个体的骨骼肌组织内的表达变化.结果表明,民猪的YB1基因完整的CDS区长975 bp,编码325个氨基酸,具有1个高度保守的冷休克域,存在多个磷酸化位点,不包含任何跨膜区域,是一种存在于细胞质中的蛋白质.组织表达谱表明YB1基因在所检测的7种组织内均高水平表达.该基因在被冷刺激13 d后,在腿肌内的表达水平出现显著下降(P＜0.05).冷刺激造成了YB1基因转录发生了变化.%This experiment was conducted to study whether Y-box binding protein participate the cold-adaptive response of mammalian. The RT-PCR and Real-time PCR methods were used to clone and sequence the YB1 gene of Min pig. The expression level of YB1 in large intestine, leg muscle, lung, fat, heart, spleen and liver was detected. The expression change between ordinary temperature group ((10±2)℃)and low temperature group ((-20±3)℃) after thirteen days cold-stimulation in skeletal muscle was compared. The results showed that the complete CDS of Min pig YB1 was 975 bp, encoding 325 amino acids, having a highly conserved cold shock domain, existing some phosphorylation sites, which didn't contain any transmembrane domain, and was a protein found in the cytoplasm. Tissue expression pattern showed that the YB1 gene was highly expressed in tissues detected. The expression level of YB 1 in leg muscle was decreased significantly after thirteen days cold-stimulation (P<0. 05). The biology role of YB1 during cold-adaptation could not be determined, but the cold-stimulation really caused changes in the YB1 gene transcription.
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