Objective Taxonomic identification and genome mining of actinobacteria strain SIIA-A16124 were performed.Methods Polyphasic taxonomy based on morphology,physiological properties,cell wall composition,and the gene sequence analysis of 16S rRNA were adopted to determine the taxonomic status of strain SIIA-A16124.The prediction of bioactive secondary metabolites of the strain SIIA-A16124 was carried out by bioinformatic analysis,the determination of antibacterial activities,and ESI-MS analysis of the crude extract.Results The results showed that the similarity of 16S rRNA gene sequences between strain SIIA-16124 and the type strain Actinokineospora inagensis NRRL B-24050T was 99％.There existed obvious differences between the model strain and the strain S IIA-A 16124 which produced spores in I SP3 medium and hydrolyzed starch.Strain SIIA-A16124 was identified as Actinokineospora sp.,and the genome of this strain contained a novel lantibiotic gene cluster.In addition,the bioactive secondary product of strain SIIA-A16124 was preliminarily identified as lantibiotic.Conclusion The ability of lantibiotic biosynthesis was found for the first time in genus Actinokineospora.%目的 对湖泊放线菌SIIA-A16124进行分类鉴定和基因组挖掘.方法 采用多相分类法对菌株的形态学、生理生化、细胞壁化学组分、16S rRNA基因序列进行测定和分类鉴定,采用基因组挖掘分析生物合成基因簇,经发酵培养、抗菌活性检测及活性产物质谱分析,推测其活性产物的化合物结构类别.结果 菌株SIIA-A16124的16S rRNA基因与菌株Actinokineospora inagensis NRRL B-24050T同源性最高为99％;菌株SIIA-A 16124在ISP3培养基上中等产孢和水解淀粉的特性与模式菌株存在明显差异;鉴定菌株SIIA-A16124为动孢菌Actinokineospora sp.,具有羊毛硫肽生物合成基因簇,其活性次级代谢产物属于羊毛硫肽类抗生素.结论 首次发现动孢菌属放线菌具有生物合成羊毛硫肽类抗生素的能力.
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