Objective To study the antifungal constituents of a marine actinomycete Y12-26.Methods The fermentation centrifugal supernatant was extracted with an equal volume of butanol.The extracts was isolated and repeatedly purified by column chromatography on silica gel,preparative TLC and semi-preparative HPLC.Their structures were elucidated by MS,1H-NMR,13C-NMR spectral analysis and compared with the data of literature.Results Three compounds were isolated and identified as salicylamide (1),iturin A-2 (2),daidzein-4',7-di-α-L-rhamnoside (3) from 28g butanol extracts.Conclusion The marine actinomycete Y12-26 can produce the compounds with many biological activities.The MIC of compound 1 against Botrytis cinerea and Pyricularia oryzae is 125μg/mL,compound 2 shows significant antifungal bioactivity against Rhizoctonia solaniis 12.5μg/mL,compound 3 against Botrytis cinereais 125μg/mL.Compound 3 is reported firstly for the inhibitory activities against plant-pathogenic fungus.%目的 研究海洋放线菌Y12-26发酵液中对几种植物病原真菌具有抑制作用的次生代谢活性物质.方法 发酵离心上清液用等体积正丁醇萃取,得到发酵液浸膏.浸膏采用正相硅胶柱色谱、制备TLC和半制备HPLC等方法进行分离纯化该菌株活性次级代谢产物,并运用MS、1H NMR和13C NMR等波谱技术并结合文献比对鉴定所分离的单体化合物.结果 从28g正丁醇萃取物中分离纯化并鉴定了3个单体活性化合物,分别为:salicylamide(1)、iturin A-2(2)、daidzein-4',7-di-α-L-rhamnoside(3).结论 海洋放线菌Y12-26能代谢产生具有多种生物活性的化合物.化合物1对Botriytis cinerea和Pyricularia oryzae的最低抑菌浓度(minimal inhibitory concentration,MIC)为125 μg/mL,化合物2有强烈的抗真菌活性,其对Rhizoctonia solani的MIC为12.5μg/mL,化合物3对Botriytis cinerea的MIC为125μg/mL.本文首次报道大豆素类化合物3抗植物病原真菌活性.
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