目的 通过研究阿奇霉素对铜绿假单胞菌Ⅰ类整合子整合酶基因intI1表达的影响,初步探讨阿奇霉素对Ⅰ类整合子的抑制机制.方法 采用荧光定量RT-PCR方法检测铜绿假单胞菌Ⅰ类整合酶基因intI1在不同阿奇霉素浓度中mRNA的表达水平,分析阿奇霉素对intI1表达的影响.结果 阿奇霉素对铜绿假单胞菌Ⅰ类整合酶基因intI1的表达抑制作用明显,Ⅰ类整合酶基因intI1mRNA随着阿奇霉素浓度的增高表达水平呈明显下降趋势.结论 阿奇霉素对Ⅰ类整合酶基因intI1表达具有明显抑制作用,提示可能与阿奇霉素对铜绿假单胞菌群体感应系统抑制机制间接相关.%Objective The mechanism of azithromycin inhibition on the class 1 integron were preliminarily explored by studying the influence of azithromycin on the class 1 integron integrase intI1 gene expression in Pseudomonas aeruginosa.Methods Using fluorescence quantitative RT-PCR to detect class 1 integrase intI1 gene of Pseudomonas aeruginosa mRNA expression level in different concentrations of azithromycin,analysis of azithromycin influence on the intI1 expression.Results Azithromycin had a significant inhibitory effect on the expression of the class 1 integrase intI1 gene of Pseudomonas aeruginosa,then the class 1 integrase intI1 gene mRNA expression level increased as the concentration of azithromycin was significantly lower than the half lethal dose (2μg/mL).And the growth of PA was significantly suppressed in AZM 4μtg/mL medium.Conclusion Azithromycin has an obvious inhibitory on the expression of the class 1 integrase intI1 gene,which suggests it may be associated with azithromycin inhibition on the quorum-sensing system ofPseudomonas aeruginosa indirectly.
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