Objective To study the production of beta-lactamases on multi- drug resistant Pseudomonas aeruginosa and provide the guideline for treatment and control of Pseudomonas aeruginosa infection in hospital Methods Manal method and Biomerieux identification system were used to indentify bacterial strainsand agar diffusion method to detect drug susceptibility. K-B susceptibility method, three-dimensional method and synergetic test were used to detect extended spectrum beta-lactamases and AmpC beta-lactalases metallo-beta-lactamases. Results Specimens ranged from the first to the third of the isolation rate of PA was 71.1%, wound sedition and pus. Among 97 strains of multi-resistant Pseudomonas aeruginosa there were 21 strains Producing ESBLs beta-lactamases, 47 strains prouducing AmpC beta-lactamases, 16 strains producing ESBLs and AmpC beta-lactamases at the same time, 9 strains of them was producing metallo-beta-lactamases. The detection rate of AmpC was the most high. Conclusions The main beta-lactamases was AmpC beta-lactamases in the multi-resistant Pseudomonas aeruginosa cultured in our area. The sensitivity rates to imipenem amikacin and ciprofloxacin had better. In order to reduce the drug-resistance strains and control the infection of Pseudomonas aeruginosa antibiotics should be used reasonably according to drug susceptibility test.%目的 了解本地区多重耐药铜绿假单胞菌产酶情况及耐药性,为临床治疗及感染控制提供依据.方法 采用手工法及法国生物梅里埃鉴定系统对菌种进行鉴定,采用K-B琼脂扩散法进行药敏实验检测,改良三维试验、协同法分别对细菌ESBLs酶、AmpC酶、金属酶进行检测,根据CLSI标准进行判读.结果 临床标本主要以痰标本为主,分离率为71.1％,其次是伤口分泌物和脓汁,97株铜绿假单胞菌中产AmpC酶47株(48.5％),单产ESBLs酶菌株21株(21.6％),同时产ESBLs酶和AmpC酶菌株16株(16.4％),产金属酶的菌株9株(9.28％),产AmpC酶菌株检出率最高.讨论 多重耐药铜绿假单胞菌以产AmpC酶为主,对亚胺培南、阿米卡星、环丙沙星敏感率较高,临床应根据药敏试验结果,合理选用抗生素,减少耐药菌株产生和控制医院感染.
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