目的：比较青海高原高山土生牦牛（ Bos grunniens）和高山迁饲黄牛（ Bos taurus domestica）、低海拔黄牛血清心肌酶谱指标的差异，探讨高山牦牛对高原环境的适应机制。方法牦牛和黄牛均经颈部放血处死样本，采集全血，制备血清，全自动生化分析仪测定天门冬氨酸氨基转移酶（aspartate aminotransferase，AST）、肌酸激酶（creatine kinase，CK）、肌酸激酶同工酶（creatine kinase isozymes，CK-MB）、乳酸脱氢酶（ lactate dehydrogenase，LDH）和α-羟丁酸脱氢酶（α-hydroxybutyrate dehydrogenase，HBDH）。结果高山土生牦牛和高山迁饲黄牛的血清AST、CK、CK-MB指标均显著高于低海拔黄牛（ P＜0.05）；与高山迁饲黄牛比较，高山土生牦牛的AST、CK较高（P＜0.05）、CK-MB、LDH和HBDH较低（P＜0.05）。提示高山迁饲黄牛在高原低氧环境中生活，造成心肌细胞损伤，细胞中各种酶进入血液，使得血清酶值显著增高，表明高山迁饲黄牛处于高原习服状态。结论高山土生牦牛的血清心肌酶谱指标与低海拔黄牛具有差异性。高山迁饲黄牛处于高原习服状态，高山土生牦牛已适应高原低氧环境。%Objective To compare the myocardial enzyme spectrum of native mountain yaks ( Bos grunniens) , alien mountain yellow cattle ( Bos taurus domestica) and low altitude yellow cattle breeding in Qinghai plateau, explove the mechanism that the native mountain yaks adapt to the plateau environment.Methods The samples were treated by cervical bleeding to death, and whole blood was collected.Then serum were prepared from whole blood.The myocardial enzymes including aspartate amino transferase, creatine kinase, creatinine kinase-MB isoenzyme, lactate dehydrogenase, α-hydroxybutyrate dehydrogenase and.Results In the native mountain yaks and in the alien mountain yellow cattle, levels of AST, CK, CK-MB were significantly higher than those in the low altitude yellow cattle (P<0.05);compared with the alien mountain yellow cattle, levels of AST,CK were significantly higher than those in the native mountain yaks (P<0.05),CK-MB,LDH and HBDH were lower(P<0.05).The results suggested that under the high altitude and hypoxia environment, myocardial cell injury occurred in the alien mountain yellow cattle, thus various enzymes penetrated through the cells into the blood circulation, lead to the higher serum enzymatic levels.Conclusion The phenomenon indicates that the alien mountain yellow cattle is in a state of high altitude acclimatization.But the native mountain yaks are well adapte to the high altitude and hypoxia environment.
展开▼
