Objective To establish an optimum DNA extraction method for Chinese traditional medical herbs in order to meet necessary for DNA barcoding research.Methods Four Chinese traditional herbs, Glycyrrhiza uralensis Fisch, Phellodendron Chinensis Cortex, Cistanche tubulosa Wight and Cistanche deserticola Ma were chosen as the experimental materials, the DNA was extracted by 6 different kinds of DNA extraction method, including the improved method of high-salt combined low-pH,the improved method of SDS,CTAB method,PVP method,PlantZol Kit and Ezup Kit, the quality of DNA was investigated by ultraviolet spectrophotometry,agarose gel electrophoresis and PCR amplification by using specific primers of ITS2 and psbA-trnH. ResuIts The quality of the DNA was better than other four kinds of methods by the improved method of high-salt combined low-pH and Ezup Kit, the value of OD260/OD280 was between 1.7 ~1.9,the yield of DNA was the highest by the PlantZol kit , followed by the improved method of high-salt combined low-pH( P <0.05 ) , but the purity of DNA was poor by the PlantZol kit.The DNA electrophoresis tests showed that the DNA integrity of Glycyrrhiza uralensis Fisch and Cistanche tubulosa Wight were better with the improved method of high-salt combined low-pH, the improved SDS method, the CTAB method and the PlantZol kit.The DNA of Phellodendron Chinensis Cortex and Cistanche deserticola Ma were extracted by the six methods appeared diffuse status in the lanes.But only the improved method of high-salt combined low-pH could make the PCR amplification of the success rate 100% by using specific primers of ITS2 and trnH-psbA.ConcIusion The DNA extraction method of high-salt combined low-pH can be used to establish the Chinese DNA barcoding which has the advantages of lower cost, simpler procedure and less time.%目的：确定能够满足中药材DNA条形码研究的最适DNA提取方法。方法以中药材甘草、黄柏、管花肉苁蓉和肉苁蓉为实验对象，采用改良高盐低pH法、改良SDS法、CTAB法、PVP法、PlantZol试剂盒及Ezup柱式试剂盒6种DNA提取方法进行DNA提取，采用紫外分光光度法、琼脂糖凝胶电泳和ITS2与psbA－trnH序列的引物PCR扩增对DNA产量及质量进行检测。结果改良高盐低pH法和Ezup柱式试剂盒提取的4种中药材的DNA质量相对较好，其OD260／OD280的值在1．7～1．9之间，PlantZol试剂盒法所提取的DNA得率相对较高，其次为改良高盐低pH法（P＜0．05），但PlantZol试剂盒所提取DNA的纯度较差，DNA电泳检测表明改良高盐低pH法、改良SDS法、CTAB法和PlantZol试剂盒所提取到甘草和管花肉苁蓉的DNA完整性较好，而6种方法所提取的黄柏与肉苁蓉的DNA则均在泳道内呈弥散状态，但只有改良高盐低pH法其ITS2和psbA－trnH序列的引物PCR扩增成功率为100％。结论改良高盐低pH值法提取的基因组DNA可以用来中药DNA条形码的建立，且该方法具有成本低、步骤简单、时间短等优点，是一种高效、快速、经济的中药材基因组DNA提取方法。
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