目的：通过对脂肪肝模型家兔注射大株红景天注射液来探求对血清瘦素及肝脏瘦素受体mRNA表达的影响。方法选取120只成年健康雄性新西兰白兔，采取随机数表法分组，正常组30只，予普通饲料喂养8周。高脂组90只，通过喂食高脂饲料4周，制备脂肪肝模型家兔，其中模型组30只，喂食高脂饲料4周；对照组30只，高脂饲料喂食，予二甲双胍格列本脲片，每只每天150 mg/kg，持续4周；实验组30只，高脂饲料喂食，予大株红景天注射液，每只每天40 mg/Kg，持续4周。试验结束后，对比各组家兔血清瘦素、肝脏瘦素水平及瘦素受体mRNA表达水平、肝指数水平。结果实验组与对照组血清瘦素及肝脏瘦素水平均不同程度提高，且实验组血清瘦素（4.51±1.17）ng/mL、肝脏瘦素（6.43±1.93）ng/mL较对照组血清瘦素（7.36±1.26）ng/mL、肝脏瘦素（12.33±3.27）ng/mL明显降低，差异有统计学意义（P＜0.05）；实验组与对照组血清瘦素及肝脏瘦素受体mRNA表达水平均不同程度提高，且实验组血清瘦素mRNA表达水平（0.98±0.15）、肝脏瘦素表达水平（1.78±0.33）较对照组血清瘦素表达水平（2.57±0.29）、肝脏瘦素表达水平（3.31±0.26）明显降低，差异有统计学意义（P＜0.05）；实验组与对照组肝指数水平均不同程度提高，且实验组肝指数（3.98±0.13）％较对照组肝指数（4.77±0.20）％明显降低，差异有统计学意义（P＜0.05）。结论大株红景天注射液能够明显降低家兔血清及肝脏的瘦素水平，并抑制瘦素受体mRNA表达，改善肝指数，对脂肪肝治疗具有指导意义。%Objective To explore the effect of big strain rhodiola injection on levels of leptin and leptin receptor mRNA in serum and liver of rabbit with fatty liver.Methods 120 healthy adult male New Zealand white rabbits were grouped with a random number table method.30 rabbits with normal diet for eight weeks in normal group;90 rabbits in fat group were fed with high fat diet for four weeks to prepare fatty liver model in which 30 rabbits were fed with high-fat diet for four weeks as model group,30 rabbits with high-fat diet and glyburide metformin tablets [150 mg/(kg·d)]for 4 weeks as control group,30 rabbits with high-fat diet and big strain rhodiola injection [40 mg/(kg·d)]for four weeks as experimental group.After the test, levels of serum leptin,hepatic leptin,expression levels of leptin receptor mRNA and levels of liver index were compared among groups.Results Serum leptin and liver leptin in experimental group and control group were all increased to varying degrees,and the serum leptin level [(4.51 ±1.17)ng/mL],liver leptin level [(6.43 ±1.93)ng/mL]of experimental group was lower than that of control group respectively,in which the serum leptin was (7.36 ±1.26)ng/mL and liver leptin was (12.33 ±3.27)ng/mL (P<0.05).Serum leptin and leptin liver receptor mRNA in experimental group and control group were all increased to varying degrees,and the serum leptin mRNA level (0.98 ±0.15),liver mRNA level (1.78 ±0.33)of experimental group was lower than that of control group,in which the mRNA level of serum leptin was (2.57 ±0.29)and liver leptin mRNA levels was (3.31 ±0. 26)(P<0.05).Levels of liver index in experimental group and control group were increased,and liver index of experimental group [(3.98 ±0. 13)%]was lower than that in control group [(4.77 ±0.20)%],and the difference was statistically significant (P<0.05).Conclusion Big strain rhodiola injection could significantly reduce leptin levels in serum and liver of rabbits,reduce expression the leptin receptor mRNA levels and improve liver index,which has guiding significance for the treatment of fatty liver.
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