Fermentation conditions of exopolysaccharides ( EPS) production by the Antarctic bacterium Pseudoalteromonas sp� 3⁃3⁃1⁃2 were optimized. The single factor experiment showed that the optimal carbon and nitrogen source were sucrose ( 40 g/L) and potassium nitrate ( 10 g/L) , respectively. The optimal temperature,salinity and initial pH of media were 15 ℃,4�5% and 9,respectively. By the Box⁃Behnken experimental design and analysis by Design⁃Expert software Response Surface, the optimized conditions of EPS production of the Antarctic bacterium Pseudoalteromonas sp. 3⁃3⁃1⁃2 were as following:sucrose,43�1 g/L, potassium nitrate 9�6 g/L,temperature 17�2 ℃. Under the optimal conditions,EPS production of strain 3⁃3⁃1⁃2 was reached 3�03 g/L.%利用单因子试验和响应面法对南极细菌Pseudoalteromonas sp�3312产胞外多糖条件进行了优化。通过单因子试验确定菌株3312产胞外多糖的最佳碳源和氮源分别为蔗糖和KNO3，其最佳添加量分别为40 g／L和10 g／L；最适培养温度为15�0℃；最佳培养基盐度和初始pH分别为4�5％和9；并确定了对产糖有显著影响的单因素———碳源（蔗糖）、氮源（ KNO3）和温度。通过Box Behnken进行试验设计和Design Expert响应面软件分析，得到了菌株3312产胞外多糖发酵条件的优化条件：蔗糖43�1 g／L、KNO39�6 g／L和温度17�2℃。在此优化条件下，菌株3312发酵液的粗胞外多糖产量可达3�03g／L。
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