以黄色短杆菌(Brevibacterium flavum)NJ-237为出发菌株,通过梯度传代适应性培养及同浓度药物平板富集培养的方式,逐步提高菌体的抗药物性能,获得了1株耐高糖和耐高浓度α-氨基丁酸(α-AB)的菌株NJ-2372.在单因素实验的基础上,利用响应面分析法对影响该菌株L-缬氨酸(L-Val)产量的3个重要因素玉米浆、生物素(VH)、硫胺素(VB1)的添加量进行优化.结果表明:当玉米浆、VH、VB1最佳添加量分别为11 g/L、35 μg/L和101 μg/L时,摇瓶发酵72 h,L-Val摇瓶发酵产量达到52.9 g/L.%Using Brevibacterium flavum NJ-237 as the original strain by gradual adaptive culture and synchronized enrichment culture on the same concentration of additives, a L-valine producing mutant NJ2372 was obtained.It was resistant to high concentration of glucose and α-aminobutyric acid.On the basis of single-factor experiments, the optimal concentrations of the three important factors including corn steep liquor, VH, VB1 were obtained by response surface methodology.The concentrations of corn steep liquor, VH, VB1 were 11 g/L, 35 μg/L, and 101 μg/L, respectively, and L-valine production of the strain was 52.9 g/L for 72 h in shaker flask.
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