Using Escherichia coli and Staphylococcus aureus as model strains, the antibacterial effect and the mechanism of silver nitrate were investigated. Experimental results indicated that the silver nitrate of 2. 891 mg/mL could inhibit completely the growth of 106cfu/mL cells in liquid LB medium, and that silver nitrate prolonged the lag phase of E. coli and S. aureus, the higher the concentration, the longer the lag phase. In addition, silver nitrate had a marked effect on the dehydrogenase activity of E. coli and S.aureus, the dehydrogenase activity decreased as the concentration increased. The surface hydrophobicity of bacterial cells all had a decline to some extent followed the solution of silver nitrate worked on the bacteria, and the higher the concentration, the more its effect. The descending degree in E. coli was larger than that in S. aureus.%以大肠杆菌和金黄色葡萄球菌为模式菌,对AgNO3的抗菌效果进行研究,并对其抗菌机制作初步探讨.AgNO3对大肠杆菌的抑制生长曲线表明:2.891 mg/L 的AgNO3能够完全抑制106 个/mL 的大肠杆菌细胞生长,AgNO3使大肠杆菌和金黄色葡萄球菌的延滞期加长,并且浓度越高,延滞期越长.另外,AgNO3对大肠杆菌和金黄色葡萄球菌脱氢酶的活性有明显影响,随着AgNO3浓度的提高,脱氢酶的活性逐渐降低.AgNO3溶液作用于细菌后,细菌表面疏水性均有不同程度地下降,且浓度越大对其影响也越明显,大肠杆菌的下降程度要大于金黄色葡萄球菌.
展开▼
