Strains of actinomycetes were screened for their carboxymethyl cellulase (CMCase) productivity.Streptomyces sp. S10 A09 was chosen to be the best producing strain.In order to obtain higher filter paper activity, Plackett-Burman and Center Composite Design of response surface methodology were used to optimize conditions of cellulase production by Streptomyces sp.S10 A09.CMC-Na and (NH4) 2SO4 were the most significant factors improving enzyme production.The Center Composite Design results showed that the optimum medium was as follows:CMC-Na 2.57 g/L, (NH4) 2SO4 11.31 g/L, KH2PO4 0.2 g/L, MgSO4 1 g/L, FeSO4 0.01 g/L.Under the above conditions, the filter paper activity was 125.96 U/mL, 3 times as that before the optimization.%在筛选纤维素酶活菌株时, 发现一株放线菌链霉菌属S10A09具有较高的纤维素酶活力.为了获得高酶活纤维素酶, 将Plackett-Burman (PB) 筛选和中心组合设计 (CCD) 以及响应面分析法相结合, 考察影响链霉菌属S10A09发酵生产滤纸酶的发酵条件.Plackett-Burman结果表明, 羧甲基纤维素钠 (CMC-Na) 和 (NH4) 2SO4是影响S10A09发酵产纤维素酶活高低的主要因素.CCD实验优化后产酶最优发酵培养基 (g/L) 为CMC-Na 2.57、 (NH4) 2SO411.31、KH2PO4 0.2、MgSO41、FeSO40.01.优化后, 滤纸酶活 (FPA) 达到125.96 U/mL, 接近优化前的3倍.
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