Objective To investigate the relationship between lens epithelial cells (LEC)apoptosis of the dia-betic cataract and senile cataract and Fas,P53 gene.Methods Anterior lens capsules specimens 45 cases were took from diabetic and senile cataract patients in the cataract ultrasonic emulsification operation,using light and electron mi-croscopic to observing apoptosis of LEC,and applying RT-PCR to detect quantitatively expression of Fas protein and Bax protein in cataract LEC.Results Two kinds of cataract in the LEC had both protein expression of Fas and P53.Fas pro-tein expression in diabetic cataract LEC was 13.34 times in senile cataract.P53 protein expression in diabetic cataract LEC was 7.3 times in senile cataract,there were significant differences (P <0.05).Conclusion Fas and P53 genes may play an important role in cataract LEC apoptosis.%目的：探讨糖尿病性白内障及老年性白内障晶状体上皮细胞（LEC）的凋亡与 Fas、P53基因的关系。方法在白内障超声乳化手术中取糖尿病性及老年性白内障患者的晶状体前囊膜标本45例，应用光镜，电镜观察 LEC 的凋亡情况，应用 RT-PCR 定量检测 Fas 蛋白，P53蛋白在白内障 LEC 中的表达。结果两种白内障 LEC 中均有 Fas，P53蛋白表达。Fas 蛋白在糖尿病性白内障 LEC 中的表达平均是老年性白内障的13．34倍， P53蛋白在糖尿病性白内障 LEC 中的表达平均是老年性白内障的7．3倍，两者差异有统计学意义（P ＜0．05）。结论两种白内障 LEC 均出现凋亡，Fas、P53基因在糖尿病性白内障 LEC 凋亡中起重要作用。
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