Objective:To explore the lipid metabolic characteristics of functionally exhausted CD8+T cells in tumor microenvironment of hepatocellular carcinoma(HCC).Methods:By using flow cytometry analysis and real-time PCR to detect the functions and lipid metabolism-related proteins and genes expression of CD8+T cells in HCC and peri-tumor tissues,then the relationship between function and lipid metabolism was analyzed.Objective:The infiltrated CD8+T cells in HCC have the following characteristics: functional exhaustion(decreased interferon-γ secretion and up regulated programmed death molecule-1 expression);down regulated expression of multiple fatty acid synthases,and the decreased content of intracellular fatty acids;impaired mitochondrial structure and function(P<0.05).Conclusions:Fatty acid metabolism abnormalities are the characteristics of exhausted CD8+T cell infiltrated in HCC.%目的:探讨肝细胞肝癌(hepatocellular carcinoma,HCC)肿瘤微环境中CD8+T细胞耗竭与脂代谢的关系.方法:利用流式细胞分析、实时细胞能量代谢检测仪、实时PCR等方法检测HCC和癌旁组织中CD8+T细胞脂代谢及功能相关蛋白或基因的表达;在此基础上分析其功能和脂代谢的关系.结果:HCC浸润的CD8+T细胞具有以下特征:功能耗竭(干扰素-γ减少、程序性死亡分子-1表达升高);多个脂肪酸合成酶表达下调,脂肪酸含量下降;线粒体结构、功能受损(P<0.05).结论:HCC中浸润的功能耗竭CD8+T细胞具有脂肪酸代谢异常特征.
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