首页> 美国政府科技报告 >Functional Characteristics of Tumor Associated Protein Spot14 and Interacting Proteins in Mouse Mammary Epithelial and Breast Cancer Cell Lines.

【24h】

Functional Characteristics of Tumor Associated Protein Spot14 and Interacting Proteins in Mouse Mammary Epithelial and Breast Cancer Cell Lines.

机译：小鼠乳腺上皮细胞和乳腺癌细胞系中肿瘤相关蛋白spot14和相互作用蛋白的功能特征。

获取原文

页面导航

摘要
著录项
相似文献
相关主题

摘要

Thyroid Hormone Responsive Protein Spot14 (S14) is necessary for high rate de novo fatty acid synthesis. Elevated S14 is correlated with reduced disease free survival of women afflicted with breast cancer. The molecular mechanism of S14 remains illusive. Two models exist for S14 function: one suggesting transcriptional events and the other in metabolic processes. Our previous findings showed that S14 does not alter lipogenic gene expression in the cell lines investigated even if driven to the nucleus. S14 overexpression did not affect glycolytic or lipogenic enzyme levels in normal or ErbB2 tumor cells. However, S14 overexpression enhanced accumulation of total lipids evaluated by Bodipy staining and NMR analysis. A major finding in this report is that glycolytic and lipogenic enzyme abundance was altered in a serum dependent manner for normal but not ErbB2 cells. We found that mouse ErbB2 cells do not respond to progestin to induce S14 because they lack the progesterone receptor (PR). In contrast, S14 was sharply induced along with FASN by progestin in T47D human breast cancer cells known to express PR. Progestin stimulated total lipid accumulation and increased total fatty acids in T47D cells. Importantly, 13C(U) glucose tracer studies showed a progestin dependent increase of 13C incorporation into de novo palmitate. Together, these results show that S14 does not alter gene expression but functions to enhance the de novo fatty acid synthesis pathway in a progestin dependent way.

著录项

作者
Rudolph, M. C.;
展开▼
作者单位

展开▼
年度 2012
页码 1-12
总页数 12
原文格式 PDF
正文语种 eng
中图分类工业技术;
关键词
Breast cancer; Cells(Biology); Progesterone; Proteins; Receptor sites(Physiology); Synthesis(Chemistry); Blood serum; Diseases; Enzymes; Epithelium; Fatty acids; Genes; Glycosidases; Lipids; Nuclei; Sense organs; Thyroid hormones; Thrsp (Spot14); Cancer metabolism; Fatty acid synthesis; Pr(Progesterone receptors);

机译：乳腺癌;细胞（生物学）;孕酮;蛋白质;受体位点（生理学）;合成（化学）;血清;疾病;酶;上皮;脂肪酸;基因;糖苷酶;脂质;细胞核;感觉器官;甲状腺激素; Thrsp （spot14）;癌症代谢;脂肪酸合成; pr（孕酮受体）;

相似文献

外文文献
中文文献
专利

1. CCAAT/Enhancer binding protein delta (c/EBPdelta) regulation and expression in human mammary epithelial cells: I. "Loss of function" alterations in the c/EBPdelta growth inhibitory pathway in breast cancer cell lines. [J] . Sivko GS, DeWille JW Journal of cellular biochemistry. . 2004,第4期

机译：CCAAT /增强子结合蛋白δ（c / EBPdelta）在人乳腺上皮细胞中的调节和表达：I.乳腺癌细胞系中c / EBPdelta生长抑制途径的“功能丧失”改变。
2. Expression and function of CCAAT/enhancer binding proteinbeta (C/EBPbeta) LAP and LIP isoforms in mouse mammary gland, tumors and cultured mammary epithelial cells. [J] . Dearth LR, Hutt J, Sattler A, Journal of cellular biochemistry. . 2001,第3期

机译：CCAAT /增强子结合蛋白β（C / EBPbeta）LAP和LIP亚型在小鼠乳腺，肿瘤和培养的乳腺上皮细胞中的表达和功能。
3. Proteomics of Mouse BRCA1 -deficient Mammary Tumors Identifies DNA Repair Proteins with Potential Diagnostic and Prognostic Value in Human Breast Cancer [J] . Marc Warmoes, Janneke E. Jaspers, Thang V. Pham, Molecular & cellular proteomics: MCP . 2012,第7期

机译：小鼠BRCA1缺陷型乳腺肿瘤的蛋白质组学鉴定DNA修复蛋白在人类乳腺癌中具有潜在的诊断和预后价值
4. 14-3-3γinteracts with eIF1AX and RPS7 to regulate milk protein translation and cell proliferation in bovine mammary epithelial cells [C] . C.P.Yu, C.M.Wang, Q.Z.Li, 4th international symposium on dairy cow nutrition and milk quality proceedings . 2015

机译：14-3-3γ与eIF1AX和RPS7相互作用以调节牛乳腺上皮细胞中的乳蛋白翻译和细胞增殖
5. Characterization of the CCAAT/enhancer binding protein-beta activator isoforms in normal mammary epithelial cells and breast cancer cells. [D] . Eaton, Erin MacDonald. 2003

机译：正常乳腺上皮细胞和乳腺癌细胞中CCAAT /增强子结合蛋白-β激活亚型的特征。
6. Proteomics of Mouse BRCA1-deficient Mammary Tumors Identifies DNA Repair Proteins with Potential Diagnostic and Prognostic Value in Human Breast Cancer [O] . Marc Warmoes, Janneke E. Jaspers, Thang V. Pham, 2012

机译：小鼠BRCA1缺陷型乳腺肿瘤的蛋白质组学鉴定具有人类乳腺癌潜在诊断和预后价值的DNA修复蛋白
7. Ku proteins interact with activator protein-2 transcription factors and contribute to ERBB2 overexpression in breast cancer cell lines. [O] . Nolens, Grégory, Pignon, Jean-Christophe, Koopmansch, Benjamin, 2009

机译：Ku蛋白与激活蛋白2转录因子相互作用，并有助于ERBB2在乳腺癌细胞系中的过表达。

Functional Characteristics of Tumor Associated Protein Spot14 and Interacting Proteins in Mouse Mammary Epithelial and Breast Cancer Cell Lines.

摘要

著录项

相似文献

相关主题

期刊订阅