目的:不同浓度的磁性纳米颗粒作用于肝癌HepG2细胞后,检测其凋亡情况,并电镜观察细胞形态变化.制备抗肿瘤靶向药物紫杉醇白蛋白磁性纳米颗粒(MAG-TAX-NP),将其联合碘化油治疗大鼠肝癌,观察治疗效果.方法:化学共沉淀法制备磁流体,将其作用于HepG2细胞,式细胞仪检测细胞凋亡情况.电镜观察磁微球作用于肝癌HepG2细胞后的形态学变化.用乳化-声加热固化法制备紫杉醇磁性纳米颗粒.建立大鼠肝癌模型,将荷瘤大鼠30只随机分为5组,除白对照组外,分别将碘化油、碘化油紫杉醇、碘化油纳米磁流体、碘化油紫杉醇白蛋白磁性纳米颗粒各0.2mL注入其余4组大鼠肝固有动脉内.紫杉醇用量为5mg/kg体重,肝肿瘤区外加磁场14d后处死大鼠,取肝肿瘤组织称重,计算各组抑瘤率.结果:证实了磁流体可诱导肝癌HepG2细胞凋亡.电镜下可见肿瘤细胞吞噬磁流体后细胞内形成凋亡小体,细胞裂解增加.大鼠肝癌模中除空白对照组外,其余各组的抑瘤率分别为43.2%、51%、57.4%、87.4%.结论:磁微球可诱肝癌HepG2细胞凋亡.碘化油紫杉醇白蛋白磁性纳米颗粒局部栓塞可抑制肿瘤生长,较其他组具有更强的抑瘤效果.随着深入研究,磁体有望成为治疗肿瘤的方法,紫杉醇白蛋白磁性纳米颗粒可作为临紫杉醇药物治疗肝癌的新剂型.%Objective: To study the rate of hepatoma cell apoptosis and the reduction in tumor size after administering paclitaxel albumin magnetic nanopartides ( MAG-TAX-NP ) in combination with iodized oil embolization. Methods: The chemical co-precipitation method was used to prepare the magnetic fluid. Cell apoptosis was determined by flow cytometry. Morphological changes in HepG2 liver cancer cells were observed using electron microscopy following exposure to magnetic microspheres. The emul si fi cation-ultrasonic-solidification heat method was used to prepare the paclitaxel magnetic nanopartides. Thirty liver tumor-bearing rats were randomly divided into 5 groups. The rats in the 4 treatment groups were injected with 0.2 mL of iodized oil, taxol iodized oil, iodized oil nanometer ferrofluid, or paditaxd albumin iodized oil magnetic nanopartides via the hepatic artery, respectively, while a control group was left untreated. The dosage of paditaxd was 5 mg/kg bodyweight, and magnetic fidds were applied over the liver tumor area. Rats from all 4 treatment groups were sacrificed 14 days later and the liver tumors were weighed and compared to the control group to calculate the inhibition ratio. Results: The induction of HepG2 cell apoptosis by magnetic fluid was confirmed by dectron microscopy. The formation of apoptotic bodies and cell lysis after phagocytosis of HepG2 cdls was also observed. In the liver tumor-bearing rats, the inhibition ratios were 43.2% ( iodized oil ), 51% ( taxol iodized oil ), 57.4% ( oil nanometer ferrofluid ), and 87.4% ( paditaxel albumin iodized oil magnetic nanopartides ). Conclusion: Magnetic microspheres can induce the apoptosis of HepG2 cdls. Paditaxd albumin iodized oil magnetic nanopartides mediated embolization of the rat hepatoma and inhibited tumor growth more strongly than iodized oil embolization alone or paclitaxel iodized oil embolization. Further advances in the use of these magneticfluids may lead to a new
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