目的 研究胃食管反流病(GERD)患者白细胞介素-1β基因(IL-1β-511)及白细胞介素-1受体拮抗剂基因(IL-1RN)多态性情况,分析GERD与IL-1β及白细胞介素1受体拮抗剂(IL-1RA)之间的相关性.方法 选取84例GERD患者(GERD组)及80例健康体检者(健康对照组),进行胃镜检查并且监测幽门螺杆菌(Hp)感染情况,提取DNA以聚合酶链式反应方法检测IL-1RN基因型,以聚合酶链式反应-限制性片段长度多态性方法检测IL-1β-511,对结果进行分析.结果 (1)GERD组Hp阳性35例(41.7%),健康对照组Hp检测阳性56例(70.0%),2组患者Hp阳性率比较差异有统计学意义(P<0.05).(2)GERD组与健康对照组IL-1β-511各基因型和等位基因频率分布差异无统计学意义(P>0.05);IL-1RN-1,2基因型、非2携带子、2携带子、等位基因2基因频率分布差异有统计学意义(P<0.05),其他各基因型组间比较差异无统计学意义(P>0.05);2组IL-1β-511/IL-1RN混合基因型T1基因型分布频率差异有统计学意义(P<0.05),其他各基因型组间比较差异无统计学意义(P>0.05).结论 IL-1β、IL-1RN基因多态性与GERD具有密切相关性,IL-1RN-1,2基因型、2携带子、等位基因2基因可能增加患者患病的风险,而IL-1β-511/IL-1RN混合基因型中T1基因型可能降低患者患病风险.%Objective To study the gastroesophageal reflux disease interleukins(31 gene ( IL-1 β-511 ) and interleukins-1 receptor antagonist gene ( IL-1RN ) polymorphism condition, and analyze correlation between esophageal reflux disease and IL-1 (3 and IL-1 RA. Methods Selected 84 cases of gastroesophageal reflux disease ( GERD group ) and 80 cases of normal healthy subjects ( control group ), they all underwent gastro-scope and monitoring of helicobacter pylori ( Hp ) infection status, and using extracted DNA polymerase chain reaction method detected IL-1RN genotypes, with the polymerase chain reaction restriction fragment length polymorphism method detected IL-1 β-511, then analyzed the results. Results ( 1 ) GERD group Hp test shows positive in 35 cases( 41.7% ),negative person in 49 cases( 59. 3% ). Control group Hp testing positive for 56 patients( 70. 0% ), negative in 24 cases( 30.0% ), the differences of Hp positive rate between the two groups were statistically significant ( P <0. 05 ). ( 2 ) There were no statistics difference of IL-1 (3-511 each genotype and allele frequency distribution between GERD group and control group ( P >0. 05 ); there were statistically significant differences of IL-1RN 1, 2 genotype, 2 to carry the son, and allele 2 gene frequency distribution between GERD group and control group ( P <0.05 ), there was statistically significant differences of other genotype between these groups ( P >0. 05 ); there were statistically significant differences of IL-11β-511/IL-1RN mixed genotype Tl genotype distribution frequency between GERD group and control group ( P < 0.05 ), the other genotype between groups was statistically significant differences ( P > 0. 05 ). Conclusion IL-1 β, IL-1RN gene polymorphism and gastroesophageal reflux disease has close correlation,IL-1 RN 1, 2 genotype, 2 to carry the son, and allele 2 gene may increase the risk for patients, and IL-1 β-511 / IL-1 RN hybrid genetic type of Tl genotype may reduce the risk of patients.
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