In situ hybridization molecular hybrid technology as a combination of the basic principle of molecular hybridization and immunohistochemical is provided with a high application value, especially digoxigenin marker, which is characterized by safety, convenient, time-saving, and superior to biotin markers and radioactive marker technology in sensitivity and quality control. In this paper, the basic principles of digoxigenin in situ hybridization was described, and the current applications of this technique in breeding and disease diagnose were discussed in aquatic animals.%原位杂交技术作为分子杂交的基本原理和免疫组织化学两者相结合的一项技术，有很高的应用价值，其中地高辛标记物凭借其系统安全、方便、省时，敏感性和质量控制优于生物素标记和放射性物质标记技术，被广大研究者认可。本文重点介绍了地高辛原位杂交的原理，概括了其在水产动物育种与疾病诊断中的应用现状。
展开▼
