目的：观察硫酸脑苷酯活化Ⅱ型NKT细胞对哮喘小鼠气道炎症的影响。方法：32只BALB/c小鼠随机分为对照组、哮喘组、硫酸脑苷酯治疗组和过继转移组，每组8只。对照组和哮喘组分别采用PBS和卵清白蛋白（ OVA）致敏和激发，硫酸脑苷酯治疗组和过继转移组采用OVA致敏，分别在激发前给予硫酸脑苷酯腹腔注射和硫酸脑苷酯活化的Ⅱ型NKT细胞尾静脉注射。分别采用HE和PAS染色检测肺组织学和气道杯状细胞；姬姆萨染色检测支气管肺泡灌洗液（ BALF）各细胞分类计数；ELISA法检测血清OVA特异性IgE和BALF中IL-4、IL-5水平；流式细胞仪检测肺Ⅱ型NKT细胞、IL-4＋和IFN-γ＋Ⅱ型NKT细胞的数量。结果：硫酸脑苷酯治疗组和过继转移组小鼠肺组织炎性细胞和气道基底膜杯状细胞减少。硫酸脑苷酯治疗组和过继转移组小鼠BALF中嗜酸细胞百分比、血清OVA特异性IgE和BALF中IL-4、IL-5水平明显低于哮喘组（均P＜0.05）。硫酸脑苷酯治疗组小鼠肺IL-4＋和IFN-γ＋Ⅱ型NKT细胞的数量明显高于哮喘组（均P＜0.01）。结论：硫酸脑苷酯可能通过活化Ⅱ型NKT细胞抑制哮喘小鼠气道炎症。%Objective:To investigate the effect of type ⅡNKT cells activated by sulfatide on airway inflammation in a murine model of asthma.Methods:Thirty-two BALB/c mice were randomly divided into four groups:normal control group ( n=8 ) , asthma group (n=8),sulfatide treatment group (n=8) and adoptive transfer group (n=8).The murine model of asthma was established by sensitization with intraperitoneal injection of ovalbumin ( OVA) and intranasal challenge in all animals except for the normal control group where PBS was used instead.Intraperitoneal injection of sulfatide in a sulfatide treatment group, adoptive transfer of sulfatide-activated typeⅡNKT cells in adoptive transfer group and PBS in asthma group were carried out 1 hour before the first challenge.PBS was used for intraperitoneal administration in the normal control group.Lung histology and goblet cell hyperplasia were analyzed by HE or PAS staining.Differential cell count in bronchial alveolar lavage ( BALF) was measured by May-Gruenwald Giemsa;levels of OVA-specific IgE in serum and L-4,IL-5 in BALF were measured by ELISA.The percentages of lung type Ⅱ NKT cells,IL-4+and IFN-γ+typeⅡNKT cells were detected by flow cytometry.Results:Inflammatory cell infiltration in lung tissue and goblet cell hyperplasia in the airway were decreased in sulfatide treatment group and adoptive transfer group.Percentages of eosinophil in BALF,level of OVA-specific IgE in serum,and levels of IL-4,IL-5 in BALF in sulfatide treatment group and adoptive transfer group were significantly lower than those in asthma group (all P<0.05).The percentages of lung IL-4+and IFN-γ+typeⅡNKT cells in sulfatide treatment group was significantly higher than those in asthma group ( P<0.01 ).Conclusion: Type Ⅱ NKT cells activated by sulfatide may inhibit airway inflammation in a murine model of asthma.
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