Objective To explore the protective effects of electro-acupuncture (EA) serum onβ-amyloid protein (Aβ) induced primary rat hippcampal neurons. Methods The rat models of Alzheimer's disease were established by intracerebral injection of Aβ1-40. After treated them with EA, the serum was harvested. Primary cultured hippocampal neurons were treated with Aβ25-35 to establish neuronal damage model in vitro, and divided into normal group, model group and EA serum group. The proliferation of neurons was detected by MTT test. Neuronal apoptosis was examined by TUNEL staining, and expression of cysteine aspartic acid proteases-3 (Caspase-3) was detected by immunocytochemical staining. Results MTT test showed that the cell viability was significantly decreased after treated with Aβ. While compared with the model group, cell proliferation of EA serum group was significantly enhanced (P<0.01). TUNEL staining showed that the number of apoptotic cells in EA serum group decreased significantly compared with the model group (P<0.01). After 48 h of Aβ treatment, Caspase-3 expression levels were significantly elevated. However, compared with the model group, the number of Caspase-3 positive cells in EA serum group was significantly reduced (P<0.01). Conclusion The EA serum could promote the proliferation of hippocampal neurons, reduce the expression of Caspase-3, counteract the neurotoxicity of β-amyloid protein, and reduce neuronal apoptosis.%目的观察电针血清对β淀粉样蛋白(Aβ)诱导的原代大鼠海马神经元的保护作用。方法通过Aβ1-40脑内注射建立阿尔茨海默病大鼠模型,予以电针治疗后,留取各组血清。Aβ25-35处理原代培养海马神经元建立体外神经元损伤模型,实验分为正常组、模型组和电针血清组,通过MTT法检测神经元的增殖,采用TUNEL染色法检测神经元的凋亡,通过免疫细胞化学染色检测Caspase-3的表达。结果 MTT检测结果发现,经Aβ处理后,细胞活力显著下降。而与模型组比较,电针血清组细胞增殖能力明显增强(P＜0.01)；TUNEL 染色结果显示,与模型组比较,电针血清组凋亡细胞数目明显减少(P＜0.01)；经Aβ处理48 h后,Caspase-3表达水平明显升高,电针血清组Caspase-3阳性细胞数目较模型组显著减少(P＜0.01)。结论电针血清能促进海马神经元的增殖,降低其 Caspase-3的表达,对抗Aβ的神经毒性,减少神经元的凋亡。
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