To construct a neuroblastoma targeting and MRI-visible nonviral vector for siRNA delivery, and to verify the targeting ability of the vector and its visibility in MRI. Methods PEG-PEI-SPION and scAbGD2 -PEG-PEI-SPION were prepared and the properties of them were detected. The binding ability of siRNA with PEG-PEI-SPION and scAbDGD2rnPEG-PEI-SPION was determined by agarose gel electrophoresis. CLSM and MRI were used to evaluate the targeting ability of scAbGD2-PEG-PEI-SPION. Results The mean diameter of PEG-PEI-SPION and scAbGD2-PEG-PEI-SPION was (60. 0+ 2. 3)nm and (90. 0±7. 8)nm, respectively. Complete retardance of siRNA migration occurred at ratio of nitrogen to phosphorus (N/P) ratios ≥2. 2. CLSM, and MRI showed that scAbGD2-PEG-PEI-SPION had the targeting ability. ConclusionrnscAbGD2-PEG-PEI-SPION can specifically transfer siRNA to NB, and the tumor targeting effect in vitro is visible by MRL%目的 制备集靶向siRNA传递及MRI显像功能于一体的多功能靶向纳米载体,并验证其对神经母细胞瘤的靶向性及MRI示踪性.方法 制备PEG-PEI-SPION,scAbGD2 -PEG-PEI-SPION并检测其基本特性.利用凝胶阻滞检测纳米载体与siRNA的复合能力；以CLSM及MRI验证纳米载体的靶向性.结果 PEG-PEI-SPION,scAbGD2-PEG-PEI-SPION平均粒径分别为(60.0±2.3)nm及(90.0±7.8)nm.氮磷比(N/P)≥2.2时siRNA与PEG-PEI-SPION,scAbGD2 -PEG-PEI-SPION完全复合.CLSM实验及MRI结果证实scAbGD2-PEG-PEI-SPION具有靶向性.结论 scAbGD2-PEG-PEI-SPION对神经母细胞瘤具有靶向传递siRNA及MRI显影的功能.
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