Objective: To prepare the aciclovir (ACV) solid lipid nanoparticles (SLN) for eye use based on the microemulsion technique. Methods: Stearic acid, glycerol monostearate were selected as oil phase, tween 80, tween 20 and soy lecithin as emulsifier,and ethanol, glycerol, sodium cholate, sodium lauric sulfate as co-emulsifier respectively to prepare the oil-in-water microemulsions.Microemulsions dispersed into water and then SLNs were generated. Influences of formulation components and preparation procedures on turbidity and stability of SLN were investigated to optimize the best formula and preparation methods. ACV-SLNs prepared by the microemulsion methods were lyophilized, and its reconstruction effect was evaluated. The fine structure ofACV-SLN surface was observed by atomic force microscope (AFM). Results: ACV-SLN suspensions containing 1.0％ lipid with the diameter less than 150 nm were rapidly prepared by dropping method with an injector and the optimal formula, which were stable within 10 d. ACV-SLN suspensions could be reconstituted with water after lyophilized. Conclusion: The preparation of ACV-SLN with microemulsion technique is rapid and simple. Toxic organic solvents (such as dichloromethane, chloroform) and complicated machines are not involved in the process. This method is suitable for the researches and the small-scale preparation of SLN.%目的 探讨64层VCT后处理技术在肝外胆管癌(ECC)诊断中的应用.方法 回顾性分析经手术(或活检)病理证实的ECC患者45例,均接受64层VCT平扫和三期增强扫描,并经MPR和CPR后处理,32例接受仿真内镜(CTVE)和MinIP.结果 45例中,上段胆管癌(HCC)20例,其中Ⅰ型4例,Ⅱ型4例,Ⅲ型6例,Ⅳ型6例;中段胆管癌(MCC)8例;下段胆管癌(DCC)17例.病变多呈轻、中度延迟强化.MPR和CPR图像均成功得到,部分MCC和DCC病例得到较满意的CTVE(17/25,68.00%)和MinIP图像(20/25,80.00%).结合轴位图像,全部肿瘤位置及范围得到直观显示并准确诊断.结论 应用各种64层VCT后处理技术(MPR、CPR、CTVE和MinIP)能够显示ECC的各种直接和间接征象,做出准确诊断.
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