目的 预测白念珠菌细胞壁蛋白Csp37的抗原表位, 分析其作为疫苗靶点的免疫原性.方法 采用生物信息学方法对Csp37蛋白的抗原表位进行预测, 利用ProtParam网络服务器分析蛋白基本理化性质, SignaIP 3.0预测信号肽, TMHMM软件预测跨膜区, GOR4在线分析蛋白二级结构, DNAStar预测分析亲水性、可塑性、表面可及性和氨基酸抗原指数, 使用在线工具ABCPred预测B细胞抗原表位, Syfpeithi预测T细胞抗原表位.最后, 综合分析B细胞和T细胞共有抗原表位.结果 预测白念珠菌细胞壁蛋白Csp37的B细胞表位9个和T细胞表位8个, 以及共有的优势抗原表位5个, 共同优势区域为:45-48, 76-78, 153-158, 222-225, 303-305位氨基酸.结论 白念珠菌细胞壁蛋白Csp37含有丰富的抗原表位, 具有诱导细胞免疫应答和体液免疫应答的潜能, 可以作为疫苗研究的新靶点.%Objective To predict the antigenic epitopes of the cell wall protein Csp37 in Candida albicans, and analyze their immunogenicity as vaccine targets.Methods The epitopes of Csp37 were predicted by bioinformatics method;and the basic physical and chemical properties of Csp37 protein were analyzed by ProtParam network server;signal peptide was predicted by SignalP 3.0server;transmembrane domain was predicted by TMHMM;secondary structure was predicted by GOR4;hydrophilicity, surface probability, flexible regions and antigenic index were predicted by DNAStar software.ABCPred and Syfpeithi were used to predict B and T cell epitopes, respectively.Finally, the prediction results of B and T cell epitopes were further analyzed to find the common ones.Results Prediction of B cell epitopes and T cell epitopes of the cell wall protein Csp37 of C.albicans were 9and 8respectively.The B&T cell common epitopes were 5, and the common dominant region were 45-48, 76-78, 153-158, 222-225, 303-305 aa.Conclusion Cell wall protein Csp37 of C.albicans is rich in antigenic epitopes, and has the potential to induce the cellular immune response and the humoral immune response, which could be used as a new target for vaccine against candidiasis.
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