Objective To design an anoxia cells culture box and to evaluate its application in neural stem cells exposed to the oxygen and glucose deprivation. Methods Alkaline pyrogallic acid solution was used to absorb oxygen in the culture box. Oxygen concentration was tested by oxygen analyser. Cell viability of neural stem cells treated with oxygen and glucose deprivation (OGD) was detected by methyl thiazolyl tetrazolium (MTT) assay. Results Alkaline pyrogallic acid solution could reduce the oxygen concentration of the anoxia box to 0. 238% ± 0.0278% whithin 10 min. Conclusion The new-designed anoxia culture box is useful for cell oxygen deprivation culture.%目的 设计一种简易实用的缺氧细胞培养盒,并探讨其使用方法及其在神经干细胞氧糖剥夺模型中的应用.方法 用碱性焦性没食子酸溶液吸收培养盒内的氧气,以测氧仪测量盒内氧气浓度,用神经干细胞作为实验细胞,检测细胞无氧后的活性.结果 本缺氧细胞培养盒,于10 min内可使培养盒内氧浓度降至0.238％±0.0278％.结论 用碱性焦性没食子酸法清除培养盒内氧气的方法可行性较好,并可应用于细胞缺氧的实验研究.
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