Objective To explore the role of lipopolysaccharide (LPS) in the expression of human β defensin 2 (hBD-2) in human nasal mucosa epithelial cells.Methods After the human nasal mucosa epithelial cells were stimulated with LPS,the expression of hBD-2 mRNA was detected by reverse transcription polymerase chain reaction (RT-PCR) and the expression of hBD-2 protein was detected by immunocytochemistry.Results The hBD-2 mRNA could be detected 2 hours after LPS stimulation and expressed in a dose-and time-dependent manner.The hBD-2 protein could be detected in cytoplasm 4 hours after LPS stimulation.Conclusions A certain dose of LPS could induce the expression of hBD-2 in human nasal mucosa epithelial cells in a dose-and time-dependent manner.%目的 观察脂多糖(LPS)诱导人鼻黏膜上皮细胞人β防御素2(hBD-2)表达的影响.方法 用LPS刺激人鼻黏膜上皮细胞,反转录聚合酶链反应(RT-PCR)检测hBD-2 mRNA的表达,免疫细胞化学检测hBD-2蛋白的表达.结果 LPS刺激2h后人鼻黏膜上皮细胞可见hBD-2 mRNA的表达,并呈剂量和时间依赖性;刺激4h后人鼻黏膜上皮细胞胞质中可见hBD-2蛋白的表达.结论 一定剂量的LPS可诱导人鼻黏膜上皮细胞hBD-2的表达,这种表达具有一定的剂量和时间依赖性.
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