Objective To investigate the expression and distribution of Ki 67 and nm23 in chronic nasopharyngitis and nasopharyngeal carcinoma ( NPC) .Then to explore the relationship between the expressions and their clinical significance of Ki67, nm23 in NPC.Methods The expression of Ki67 and nm23 were examined by immunohistochemical assay(SP method)in 23 cases of nasopharyngitis and 59 cases of NPC.A statistical analysis was done between the expression of them and the common clinical gastric parameters ,such as clinical stage ,cervical lymph node metastasis status , distant metastasis and 5 years survival rate .Results ①The positive expression rates of Ki 67 in NPC was significantly higher than in chronic nasopharyngitis ,the positive expression rate of nm 23 in NPC was significantly lower than in chronic nasopharyngitis (P<0.01).②The positive expression of Ki67 in NPC was positive related with clinical stage, distant metastasis and 5 years survival rate.The 5 years survival rate in Ki67 positive cases were lower than that of the negative cases .③Positive expression of nm23 in NPC was negative related with clinical stage , cervical lymph node metastasis status, distant metastasis,but not with age and T stage.The 5 years survival rate in nm23 positive cases was higher than those of the negative cases .④The expression of Ki67 and nm23 in NPC had a correlation.Through the analysis of the survival curve , the patients with low expression of Ki 67 and high expression of nm23 show longer survival time than those with high of Ki 67 and low expression of nm23.Conclusions The expression of Ki67,nm23 were closely related to progression ,invasiveness and metastasis in NPC .The expression of Ki67,nm23 are helpful markers to estimate the biology action and prognosis of NPC .(Chin J Ophthalmol and Otorhinolaryngol ,2015,15:20-24)%目的：通过观察Ki67、nm23蛋白在鼻咽慢性炎症、鼻咽癌组织中的表达，探讨Ki67、nm23与鼻咽癌临床病理特征的相关性。方法采用免疫组织化学方法（ SP法）检测23例鼻咽慢性炎症组织和59例鼻咽癌病理组织中Ki67、nm23的表达情况，再对Ki67、nm23的不同程度表达与鼻咽癌患者的临床分期、淋巴结转移、远处转移及5年生存率之间的关系进行统计学分析。结果①与鼻咽黏膜慢性炎症组相比，鼻咽癌患者中Ki67的阳性表达率较高，nm23阳性表达率较低，差异有统计学意义（P＜0．01）。②鼻咽癌患者中Ki67阳性表达与临床分期及远处转移之间呈正相关；生存期＞5年的患者Ki67阳性率低于生存期＜5年的患者。③鼻咽癌患者nm23基因的阳性表达与临床分期、颈淋巴结转移及远处转移之间呈负相关；生存期＞5年的患者nm23阳性率高于生存期＜5年的患者。④Ki67与nm23两者的表达有相关性。通过生存曲线分析发现，Ki67蛋白低表达同时nm23蛋白高表达患者比Ki67蛋白高表达同时nm23蛋白低表达患者的生存时间更长。结论鼻咽癌中Ki67、nm23的表达与肿瘤的浸润、转移密切相关，两者有望成为评估预后的相关指标。（中国眼耳鼻喉科杂志，2015，15：20-24）
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