AIM:To investigate the possible mechanism of resveratrol(Res)on tumor necrosis factor-α (TNF-α)-induced monocyte chemoattractant protein-1(MCP-1)expression in primary rat pulmonary artery endothelial cells(RPAECs).METHODS: RPAECs were randomly divided into 4 groups: control group, solvent(1% DMSO) group,TNF-αgroup and Res group.Each group was divided into 1 h,4 h and 8 h subgroups(n=6 per time point).The TNF-α+C1142(a rodent chimeric mAb that neutralizes rat MCP-1)group was set up at the 8 h time point.At each time point,the protein and mRNA expression of MCP-1 was measured by Western blot and real-time PCR.RESULTS: Pre-treatment of the RPAECs with C1142 significantly down-regulated the expression of MCP-1(P<0.05).The protein and mRNA expression of MCP-1 was markedly increased in TNF-αgroup(P<0.05).Notably,incubation with Res down-re-gulated the protein and mRNA expression of MCP-1,which was significantly lower than that in TNF-αgroup(P<0.05). CONCLUSION:MCP-1 was involved in the process of TNF-α-induced injury of RPAECs.Res down-regulates the expres-sion of MCP-1 in RPAECs,thus attenuating cell injury.%目的:探讨中药单体白藜芦醇(resveratrol,Res)对肿瘤坏死因子α(tumor necrosis factor-α,TNF-α)诱导的离体大鼠肺动脉内皮细胞(rat pulmonary artery endothelial cells,RPAECs)中单核细胞趋化蛋白1(monocyte chemoattractant protein-1,MCP-1)的作用.方法:采用组织贴块法培养RPAECs,随机分为空白对照组(control组)、溶剂对照组(solvent组)、TNF-α(10 μmol/L)组和Res(50 μmol/L)+TNF-α组(Res组),每组又分成1、4和8 h三个时点(n=6),在8 h增加TNF-α+C1142(MCP-1特异性中和抗体)组(C1142组).采用Western blot 方法检测RPAECs中MCP-1蛋白表达,real-time PCR方法检测MCP-1 mRNA表达.结果:C1142可显著减少TNF-α诱导的RPAECs凋亡(P<0.05).相同时点solvent 组的MCP-1蛋白和control组比较差异无统计学显著性;TNF-α组的MCP-1蛋白及mRNA较control组增高(P<0.05);Res组的MCP-1蛋白及mRNA表达较TNF-α组降低(P<0.05).结论:MCP-1参与TNF-α诱导的RPAECs损伤;Res可降低TNF-α诱导的RPAECs中MCP-1表达,从而减少内皮细胞损伤.
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