AIM:To investigate the regulatory effects of phosphatylinositol 3-kinase/protein kinase B (PI3K/PKB) signaling pathway on the expression of osteopontin ( OPN) in transforming growth factor-β1 ( TGF-β1 )-induced hu-man hepatic stellate cells .METHODS:Human hepatic stellate cell line LX-2 was cultured in DMEM and stimulated by TGF-β1 at the final concentration of 2.5, 5, 10 and 20 μg/L for 24 h or at final concentration of 10 μg/L for 12 h, 24 h and 48 h.LX-2 cells were pretreated with wortmannin , a specific inhibitor of PI3K/PKB signaling pathway , at final con-centration of 0.1 μmol/L for 1 h, followed by incubation with TGF-β1 at final concentration of 10μg/L for 24 h.The cells were collected.The expression of OPN was detected by real-time PCR and Western blotting .RESULTS: In LX-2 cells, the expression of OPN was apparently elevated when incubated with TGF-β1 .With the increase in TGF-β1 concentration or the extension of incubation hours , the expression of OPN was increased gradually in a dose-and time-dependent manner with certain limits.LX-2 cells pretreated with wortmannin and incubated with TGF-β1 had a significant decrease in the OPN expression as compared with control group (P<0.01).CONCLUSION:The expression of OPN in TGF-β1-induced LX-2 cells is regulated by the PI3K/PKB signaling pathway.%目的：研究磷脂酰肌醇3-激酶／蛋白激酶B（PI3K／PKB）信号通路在转化生长因子β1（TGF-β1）诱导人肝星状细胞表达骨桥蛋白（ OPN ）的调控作用。方法：体外培养LX-2人肝星状细胞株，予TGF-β1（终浓度2.5、5、10、20μg／L）刺激24 h或予TGF-β1（终浓度10μg／L）刺激12 h、24 h、48 h；先经PI3K／PKB信号通路特异性抑制剂wortmannin（0.1μmol／L）预处理1 h，再予10μg／L TGF-β1刺激24 h，收集细胞，采用real-time PCR及West-ern blotting法检测OPN表达情况。结果：TGF-β1能够促进LX-2细胞表达OPN，在一定浓度和时间范围内，其表达量随着TGF-β1浓度和时间的增加而增加，呈剂量和时间依赖性关系；经wortmannin预处理再予TGF-β1刺激的LX-2细胞，与对照组相比，OPN表达受到明显抑制（ P＜0.01）。结论：TGF-β1对LX-2人肝星状细胞OPN表达具有诱导作用，此作用可能受PI3 K／PKB信号通路的调控。
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