[ ABSTRACT] AIM:To study the effect of thrombopoietin ( TPO) on chemical hypoxia-induced apoptosis of the Rattus norvegicus adrenal pheochromocytoma (PC12) cells.METHODS:The cultured PC12 cells were randomly divided into normal control group, cobalt chloride ( CoCl2 ) group, CoCl2 +TPO group and TPO group.The cell viability was mea-sured by MTT assay.The effect of TPO on CoCl2-induced cell apoptosis was analyzed by flow cytometry with Annexin V/PI double staining.The intracellular reactive oxygen species ( ROS) were detected by fluorescence microscopy, and the chan-ges of the mitochondrial membrane potential ( MMT) were determined by flow cytometry and fluorescence microscopy.RE-SULTS:Chemical oxygen agent CoCl2 significantly inhibited the growth of PC12 cells (P<0.01).The apoptotic rate in CoCl2 group was obviously higher than that in control group ( P<0.05) , while the apoptotic rate in CoCl2 +TPO group was obviously lower than that in CoCl2 group (P<0.05).TPO decreased the production of ROS, and inhibited the decrease in MMP induced by CoCl2(P<0.01).CONCLUSION: TPO has a protective effect against CoCl2-induced apoptosis of PC12 cells by decreasing the production of ROS and inhibiting the decrease in MMP.%目的：研究促血小板生成素（ TPO）对化学性缺氧诱导的大鼠嗜铬细胞瘤PC12细胞凋亡的影响及保护作用。方法：将PC12细胞进行相应实验处理，分为对照组、氯化钴（ CoCl2）处理组、CoCl2＋TPO组及TPO对照组。检测各组PC12细胞的存活率并用Annexin V／PI双染流式细胞术分别检测细胞凋亡率，线粒体膜电位的变化及细胞内活性氧簇的变化。结果：化学性缺氧模拟剂CoCl2可以明显抑制PC12细胞的生长（ P＜0.01）；与对照组比较，CoCl2组的细胞凋亡率明显升高（P＜0.05），而CoCl2＋TPO组的细胞凋亡率显著低于CoCl2组（P＜0.05）；TPO能减少细胞内活性氧簇生成以及抑制细胞线粒体膜电位的降低（P＜0.01）。结论： TPO能对抗CoCl2缺氧所致的细胞凋亡，稳定线粒体膜电位，发挥细胞保护作用。
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