目的:探讨氯沙坦钾对2型糖尿病肾病大鼠转化生长因子β1 (transforming growth factor beta 1,TGF-β1)、CD68(巨噬细胞标记物)和单核细胞趋化蛋白-1(monocyte chemoattractant protein-1,MCP-1)表达的影响,进一步说明氯沙坦钾对2型糖尿病大鼠肾脏的保护作用.方法:雄性SD大鼠30只,按随机数字表法分为正常对照组、模型组和治疗组,每组10只.15周后,观测肾组织形态学、肾功能和24 h尿蛋白定量等指标的变化,用免疫组化法检测肾脏TGF-β1、CD68和MCP-1表达水平的变化.结果:(1)与正常对照组相比,模型组及治疗组大鼠体重均降低,血糖、甘油三酯、胆固醇及MCP-1、CD68、TGF-β1的阳性细胞数升高,模型组24 h尿蛋白和肌酐升高;(2)与模型组相比,治疗组血肌酐、24 h尿蛋白、甘油三酯及MCP-1、CD68、TGF-β1的阳性细胞数明显降低.结论:在2型糖尿病肾病大鼠中氯沙坦钾可以通过减少肾组织MCP-1表达,阻止巨噬细胞的浸润,下调TGF-β1表达,对糖尿病肾病病变起到保护作用.%AIM: To observe the effects of losartan potassium on renal expression of transforming growth factor beta 1(TGF - β1 ) , CD68 and monocyle chemoattractant protein - 1 ( MCP - 1) in type 2 diabelic nephropathy rats for exploring the protective mechanism of losartan polassium on Lype 2 diabelic rat kidney. METHODS; Thirty Sprague - Daw-ley rals were randomized into 3 groups: normal conlrol group, model group and trealmenl group. The morphology of kidney tissues, the renal function, and the change of 24 h urinary prolein quanlilalive index were measured after 15 weeks of trealmenl, while TGF - β1 , CD68 and MCP - 1 expression in kidney cortex was observed by immunohislochemislry. RESULTS; Compared with the normal conlrol rals, the body weighl of the rals was lower in other groups, but the levels of blood glucose, triglyceride and cholesterol were higher. The expression of CD68, MCP - 1 and TGF - β1 , 24 h urinary prolein quanlilalive index and serum crealinine were higher in model group than those in normal conlrol rals. However, compared wilh model group, serum crealinine, 24 h urinary prolein quanlilalive index and the expression of CD68, MCP -1 and TGF - β1, were decreased in trealmenl group. CONCLUSION; Losarlan polassium prolecls the kidney of diabelic nephropalhy rals through inhibiting the expression of TGF - β1 and MCP - 1 in the kidney and reslraining macrophage infillra-tion.
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