目的:了解引起我院慢性细菌性前列腺炎的病原菌分布及其耐药谱特征,为临床诊断和治疗细菌性前列腺炎提供依据.方法:用无菌方法采集前列腺炎患者的前列腺液进行细菌培养,利用VITEK(R)2 Compact生物鉴定系统对细菌培养阳性的菌株进行种属鉴定,参照美国临床和实验室标准协会(CLSI)推荐的方法,采用K-B法进行体外药敏试验,用WHONET 5.6软件分析病原菌的耐药谱特征.结果:121例细菌性前列腺炎患者中,革兰阳性细菌感染患者93例(76.8%),革兰阴性细菌28例(23.2%),其中分离率前3位的细菌分别是金黄色葡萄球菌(29例,23.9%)、溶血性葡萄球菌(23例,19.0%)和表皮葡萄球菌(18例,14.9%).药敏试验结果显示,3种主要革兰阳性细菌对万古霉素、替考拉宁和利奈唑胺全部敏感,对庆大霉素、红霉素、克林霉素、头孢西丁、苯唑西林和环丙沙星存在不同程度的耐药(7.1%～78.4%),对青霉素的耐药率高达90%以上;大肠埃希菌对亚胺培南、美罗培南和厄他培南全部敏感,对头孢噻肟、头孢曲松、环丙沙星、四环素、复方新诺明等抗生素有不同程度的耐药(14.5% ～69.7%).结论:我院慢性细菌性前列腺炎患者的病原菌主要是金黄色葡萄球菌、溶血性葡萄球菌、表皮葡萄球菌和大肠埃希菌.慢性细菌性前列腺炎患者的治疗应以药敏试验结果为依据,合理选用抗菌药物.%AIM: To investigale the distribution and the antimicrobial resistance of the pathogenic bacteria from chronic baclerial prostatitis. METHODS: Proslalic fluid was collected under slerile condition and was inoculated for baclerial culture. Positive results of baclerial cullure were identified by VITEK(R) 2 Compact. Drug sensilivily test was carried out with K - B method according to the recommendalion of Clinical and Laboratory Standards InsLituLe( CLSI). WHO-NET 5. 6 software was used Lo analyze the resulls of drug resislance. RESULTS; Bacleria were idenlified in 121 palienls wilh chronic proslalilis, most of which were Staphylococcus aureus (29 slrains, 23. 9% ) , Staphylococcus haemolyticus (23 slrains, 19. 0% ) and Staphylococcus epidermidis ( 18 slrains, 14. 9% ) . There were 93 slrains of Gram - posilive bacleria (76. 8% ) , and Gram - negalive bacleria were 28 slrains (23. 2% ). The resulls of drug sensilivily test indicated that Staphylococcus aureus, Staphylococcus haemolyLicus and Staphylococcus epidermidis were susceptible lo vancomycin, teico-planin and linezolid, while they were resislanl lo genlamicin, erylhromycin, clindamycin, cefoxilin, oxacillin and ciproflox-acin wilh the resislanl rales from 7.1% lo 78. 4% . Escherichia coli were susceptible lo imipenem, meropenem and erlapen-em, while they were resislanl lo cefolaxime, ceftriaxone, ciprofloxacin, tetracycline and trimelhoprim - sulfamelhoxazole wilh different resislant rales ( 14. 5% ~69. 7% ) . CONCLUSION; The major pathogenic profile of chronic baclerial proslalilis was Staphylococcus aureus, Staphylococcus haemolyLicus, Staphylococcus epidermidis and Escherichia coli. Drugs should be appropriately used on chronic baclerial proslalilis palienls base on the resulls of cullure and drug sensilivily lesl.
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