目的:研究人参皂苷Rg3对HT-29结肠癌细胞株细胞增殖及对MMP-9和TIMP-1表达的影响,探讨其抑制肿瘤细胞侵袭、转移的机制.方法:分别用低剂量(25 μg/mL)、中剂量(50 μg/mL)和高剂量(100 μg/mL)人参皂苷Rg3及对照(0 μg/mL,DEME培养液和DMSO助溶剂)培养HT-29细胞24 h、48 h、72 h,采用RT-PCR方法检测MMP-9 mRNA和TIMP-1 mRNA表达,MTT法检测肿瘤细胞增殖的抑制率.结果:人参皂苷Rg3可以抑制HT-29细胞MMP-9 mRNA的表达,抑制作用随着剂量的增加及时间的延长而增强;可以促进HT-29细胞TIMP-1 mRNA的表达,促进作用随着剂量的增加及时间的延长而增强;对HT-29细胞生长的抑制作用与随着药物浓度的增加及作用时间的的延长而增强.结论:人参皂苷Rg3抑制HT-29结肠癌细胞株MMP-9的表达,促进TIMP-1的表达,并具有抑制肿瘤细胞生长的作用.%Objective To study the effect of ginsenoside Rg3 on expression of MMP-9 mRNA and TIMP-1mRNA after cultivated with HT-29 colon cancer cell line and the effect on the inhibitory effect on cell growth, and to approach the mechanism of inhibition on tumor cell s infiltration and migration. Methods Cultivating HT-29 colon cancer cell in vitro with low-dose (25 ug/mL),middle-dose (50 ug/mL) and high-dose (100 ug/mL) ginsenoside Rg3 for 24 hours,48 hours and 72 hours. The expression of MMP-9 mRNA and of TIMP-1 mRNA were detected by RT-PCR method.The inhibitory rate of cell growth was detected by the MTT method. Results Ginsenoside Rg3 could inhibit the expression of MMP-9 mRNA in HT-29 colon cancer cell, the inhibitory effect enhanced with increasing dose and time. Ginsenoside Rg3 could synergist the expression of TIMP-1 mRNA in HT-29 colon cell, the synergistic effect enhanced with increasing dose and time. With the dose increasing and time lasting,the inhibitory rate of cell growth enhanced after ginsenoside Rg3 cultivating with HT-29 colon cancer cell. Conclusion Ginsenoside Rg3 can inhibit the expression of MMP-9 mRNA and synergist the expression of TIMP-1 mRNA. Ginsenoside Rg3 can inhibit HT-29 colon cancer cell growth.
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