背景:研究证实, 二氧化钛涂层可提高种植体表面的可黏附性, 促进成骨细胞增殖和其他细胞功能, 提高周围骨与植入体的骨整合能力, 但将其作为涂层用于内固定植入预防螺钉松动的研究尚少.目的:制备二氧化钛涂层钛螺钉, 观察其用于骨内固定对于减少螺钉松动的影响.方法:通过阳极氧化法在普通钛螺钉表面制备二氧化钛涂层.将30只雄性新西兰大耳白兔 (上海市普陀区中心医院中心实验室提供) 随机分为2组, 每组15只:实验组右侧胫骨植入二氧化钛涂层钛螺钉, 对照组右侧胫骨植入普通钛螺钉, 植入后1 d、1周、4周, 取钉道周围骨组织, RT-PCR检测血小板衍生生长因子与血管内皮生长因子基因表达;植入后4周, 取钉道周围骨组织, 进行苏木精-伊红染色.结果与结论: (1) 苏木精-伊红染色显示, 对照组钉道内可见炎性细胞浸润, 部分钉道螺纹被破坏、吸收;实验组钉道螺纹清晰, 无破坏及吸收现象, 钉道内炎性细胞渗出较少; (2) RT-PCR检测显示, 两组植入1d的血小板衍生生长因子与血管内皮生长因子基因表达比较无差异 (P> 0.05);实验组植入1, 4周的血小板衍生生长因子与血管内皮生长因子基因表达均高于对照组 (P <0.05或P <0.01); (3) 结果表明, 二氧化钛涂层钛螺钉生物相容性强于普通钛螺钉, 用于内固定可有效预防螺钉松动.%BACKGROUND: Studies have confirmed that titanium dioxide coating can improve the adhesion of implant surface, promote osteoblast proliferation and other cell functions, and improve the bone integration ability between the surrounding bone and implant. But little has been done in the use of titanium dioxide coating as a coating for internal and external fixation to prevent screw loosening. OBJECTIVE: To prepare titanium dioxide coated titanium screws and to observe the effect of titanium dioxide coated titanium screws used for bone fixation to reduce screw loosening. METHODS: Titanium dioxide coating was prepared on the surface of titanium screw by anodic oxidation. Thirty male New Zealand white rabbits (provided by the Central Laboratory of Shanghai Putuo District Central Hospital in China) were randomly divided into two groups (n=15 per group). Titanium dioxide coated screws were implanted in the right tibia of the rabbits in the experimental group, and ordinary titanium screws were implanted in the right tibia of the rabbits in the control group. Bone tissue around the screw canal was taken at 1 day, 1 and 4 weeks after implantation, and levels of platelet-derived growth factor and vascular endothelial growth factor genes were detected using RT-PCR. Four weeks after implantation, bone tissue around the nail canal was taken for hematoxylin-eosin staining. RESULTS AND CONCLUSION: (1) The results of hematoxylin-eosin staining showed that inflammatory cell infiltration was seen in the screw canal of the control group, and some screw canal threads were destroyed and absorbed. In the experimental group, the screw threads were clear and had no damage or absorption; inflammatory cells in the screw canal were less exuded. (2) RT-PCR analysis revealed no significant difference in the expression of platelet-derived growth factor and vascular endothelial growth factor gene between the two groups at 1 day after implantation (P> 0.05), while the expression of platelet-derived growth factor and vascular endothelial growth factor gene in the experimental group was significantly higher than that in the control group at 1 and 4 weeks after implantation (P < 0.05 or P < 0.01). Therefore, titanium dioxide coated titanium screws are more biocompatible than common titanium screws and can effectively prevent screw loosening when used for internal fixation.
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