目的：对送检标本中分离的致病菌分布及耐药趋势进行分析，旨在做好防控措施和指导临床合理使用抗菌药物。方法进行常规培养分离鉴定，药敏试验应用K-B纸片扩散法，按CLSI/NCCLS规定的标准进行。结果3年共分离到1071株细菌，其中G＋菌414株，占38．65％，G-菌465株，占43．41％。 MRSA检出率明显上升，分别为：3％、9％、13％。纳入目标性监测的多重耐药菌（MDRO）包括：耐甲氧西林金黄色葡萄球菌（MRSA）20株、多重耐药的大肠埃希菌25株、多重耐药的肺炎克雷伯菌4株、耐万古霉素肠球菌（ VRE）1株、多重耐药的铜绿假单胞菌1株。2013年金黄色葡萄球菌开始出现万古霉素耐药株，耐药率为16％。结论妇幼专科医院病原菌群相比综合医院病种少，病原菌也相对较少，但是病原菌群也同样随着时间推移在不断变迁，耐药性逐年增长，加强监测防控，指导临床合理用药也很有意义。%Objective To analyze the distribution of pathogenic bacteria isolated from specimens and the drug resistance tendency , so as to make prevention and control and guide rational use of antimicrobial drugs .Methods Conventional culture isolation , identification and susceptibility testing were done with K-B disk diffusion method according to standard CLSI /NCCLS requirement .Results In these three years totally 1 071 bacteria were isolated, including 414 G+strains (38.65%) and 465 G-strains (43.41%).The positive rate of MRSA increased significantly , and they were 3%, 9%and 13%, respectively .The multiple resistant bacteria ( MDRO) with targeted monitoring included methicillin-resistant staphylococcus aureus (MRSA, 20 strains), multi-drug resistant Escherichia coli (25 strains), multi-drug resistant klebsiella pneumoniae ( 4 strains ) , vancomycin-resistant enterococci ( VRE, 1 strain ) and multi-drug resistant pseudomonas aeruginosa aeromonas ( 1 strain ) .In 2013 vancomycin resistant strains were found in staphylococcus aureus , and the resistant rate was 16%.Conclusion Compared with comprehensive hospitals , the pathogen groups and pathogens are less in maternal and child hospitals . However, the pathogen groups constantly change over time and drug resistance grows year after year .Strengthening monitoring and prevention to guide rational drug use in clinics is also important .
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